| 货号 | 4744S |
| 反应种属 | Mouse |
| 来源宿主 | Mouse |
| 应用 | IHC-P/IF-IC/F |
| 使用方法 | IHC-P(1:100) F (1:1600) IF-IC (1:1000) |
| 供应商 | CST |
| 背景 | SSEA-1 antibody detects a lactoseries oligosaccharide antigen that is expressed on the surface of mouse embryonal carcinoma and embryonic stem cells (1). This antigen is also found on early mouse embryos and both mouse and human germ cells, but is absent on human embryonic stem cells and human embryonic carcinoma cells. Expression of SSEA1 in these human cell types increases upon differentiation, while on the mouse cell types differentiation leads to decreased expression (2). |
| 存放说明 | -20C |
| 计算分子量 | N/A |
IHC analysis of paraffin-embedded mouse embryo, showing staining of hair follicles, using SSEA1 (MC480) Mouse mAb.使用SSEA1 (MC480)鼠源单克隆抗体对石蜡包埋的小鼠胚胎进行免疫组化分析，显示毛发滤泡。 | |
Confocal immunofluorescent analysis of mouse embryonic stem cells growing on mouse embryonic fibroblast (MEF) feeder cells using SSEA1 (MC480) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).生长在小鼠胚胎成纤维细胞（MEF）作为滋养层的小鼠胚胎干细胞使用SSEA1 (MC480)鼠源单克隆抗体（绿色）进行激光共聚焦荧光分析。用DY-554鬼笔环肽（红色）标记肌动蛋白丝。蓝色假色=DRAQ5® #4084 (荧光DNA染料). | |
Flow cytometric analysis of unpermeabilized NIH/3T3 cells (blue) and unpermeabilized F9 cells (green) using SSEA1 (MC480) Mouse mAb.使用SSEA1 (MC480) 鼠源单克隆抗体对不可透化的NIH/3T3细胞（蓝色）和不可透化的F9细胞（绿色）进行流式细胞分析。 |
