| 货号 | 4396S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Mouse |
| 应用 | W/IP/IF-F |
| 目标/特异性 | STEP (23E5) Mouse mAb detects endogenous levels of total STEP46 and STEP61 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-F (1:100) |
| 供应商 | CST |
| 背景 | Striatal enriched phosphatase (STEP, also known as PTPN5), is a protein tyrosine phosphatase expressed in dopaminoceptive neurons of the central nervous system (1). Alternative splicing produces the cytosolic STEP46 and the membrane-associated STEP61 isoforms of STEP. Dopamine activates D1 receptors and PKA, which in turn phosphorylate both isoforms of STEP. Phosphorylation of STEP61 occurs at Ser160 and Ser221, while STEP46 is phosphorylated at Ser49 (equivalent to Ser221 of STEP61) (2). NMDA-mediated activation of STEP is an important mechanism for regulation of ERK activity in neurons (3). Furthermore, STEP is involved in the regulation of both NMDAR and AMPAR trafficking (4,5). Due to its importance in cognitive function, STEP may play a role in Alzheimer disease (1). |
| 存放说明 | -20C |
| 计算分子量 | 46, 61 |
Western blot analysis of extracts from adult rat and fetal rat brain using STEP (23E5) Mouse mAb. Western blot分析成年大鼠及其胎鼠大脑细胞提取物，使用的抗体是STEP (23E5)Mouse mAb 鼠单抗。 | |
Confocal immunofluorescent analysis of rat hippocampus (left) and striatum (right) using STEP (23E5) Mouse mAb (green) and GFAP (GA5) Mouse mAb (Alexa Fluor® 647 Conjugate) #3657 (blue). Red = propidium iodide (fluorescent DNA dye). 共聚焦免疫荧光分析大鼠海马（左）、纹状体（右），使用STEP (23E5)Mouse mAb 鼠单抗（绿色）和GFAP (GA5) Mouse mAb (Alexa Fluor? 647 Conjugate) #3657（蓝色）进行标记。红色=Propiduim iodide (荧光DNA染料)。 |
