| 货号 | 5668S |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | STIM1 (D88E10) Rabbit mAb recognizes endogenous levels of total STIM1 protein. The antibody does not cross-react with STIM2. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | Ca2+ is a key second messenger in many intracellular signaling pathways. Ca2+ signals control many cellular functions ranging from short-term responses such as contraction and secretion to longer-term regulation of cell growth and proliferation (1,2). Stromal interaction molecules (STIMs) function as Ca2+ sensors that detect changes in Ca2+ content in intracellular Ca2+ stores (3). STIM1 is conserved, ubiquitously expressed, and functions as an endoplasmic reticulum (ER) Ca2+ sensor that migrates from the ER Ca2+ store to the plasma membrane where it activates calcium-release-activated calcium (CRAC) channels when the ER Ca2+ store is low (4). STIM1 is a potential tumor suppressor; defects in STIM1 may cause rhabdomyosarcoma and rhabdoid tumors (5). STIM1 can either homodimerize or form heterodimers with STIM2. STIM2 possesses a high sequence identity to STIM1 and can function as an inhibitor of STIM1-mediated plasma membrane store-operated Ca2+ entry (6). However, further investigation is required to elucidate the true physiological function of STIM2. |
| 存放说明 | -20C |
| 计算分子量 | 85 |
Confocal immunofluorescent analysis of COS-7 cells, untreated (left) or treated with histamine and thapsigargin (right) using STIM1 (D88E10) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对COS-7细胞，未处理（左）或组胺和thapsigargin处理（右）使用STIM1(D88E10)兔单克隆抗体（绿色）进行共聚焦免疫荧光分析。蓝色伪彩=DRAQ5® #4084(荧光DNA染料)。 | |
Western blot analsyis of extracts from various cell lines using STIM1 (D88E10) Rabbit mAb. 对多个细胞系使用STIM1 (D88E10)兔单克隆抗体进行Western blot分析。 |
