| 货号 | 4265S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Mouse |
| 应用 | W/IF-F |
| 目标/特异性 | STOP (175) Mouse mAb detects endogenous levels of total N-STOP and O-STOP proteins. |
| 使用方法 | WB(1:1000) IF-F (1:200) |
| 供应商 | CST |
| 背景 | Stable Tubule Only Polypeptide (STOP) is a microtubule-associated protein, and its microtubule-stabilizing activity is regulated by calmodulin (1-2). STOPs have several tissue- and developmental-specific isoforms that are encoded by a single gene. Neurons express N-STOP (exons 1-4) and E-STOP (exons 1-3), fibroblasts express F-STOP (exons 1-2), oligodendrocytes express O-STOP, and astrocytes A-STOP (3). STOPs are the major contributors stabilizing microtubules that resist depolymerization due to cold or depolymerizing drugs. STOP knock-out mice display impaired synaptic plasticity associated with severe behavioral disorders in contrast to the anticipated neuronal development and brain anatomy defects (4). |
| 存放说明 | -20C |
| 计算分子量 | 120 |
Western blot analysis of extracts from rat brain using STOP (175) Mouse mAb. Western blot分析大鼠大脑提取物，使用的抗体是STOP (175) Mouse mAb 鼠单抗。 | |
Confocal immunofluorescent analysis of rat cerebellum (left) and forebrain (right) using STOP (175) Mouse mAb (green) and MAP2 Antibody #4542 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析大鼠小脑（左）和前脑（右），使用STOP (175) Mouse mAb 鼠单抗（绿）和MAP2 Antibody 兔多抗 #4542 （红）进行标记。蓝色伪彩=DRAQ5 ® #4084(荧光DNA染料)。 | |
Western blot analysis of extracts from wild type (WT) and N-STOP knock-out mouse brain lysates, using STOP (175) Mouse mAb (upper) or Actin Antibody (lower). (Kindly provided by Dr. Robert Margolis, Sidney Kimmel Cancer Center, San Diego, California). Western blot分析野生型（WT）和N-STOP敲除小鼠大脑提取物，使用的抗体是STOP (175) Mouse mAb 鼠单抗（上图）和Actin Antibody 兔多抗（下图）。（由Sidney Kimmel Cancer Center, San Diego, California的Dr. Robert Margolis提供） |
