| 货号 | 15729S |
| 反应种属 | All |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Simian Virus 40 (SV40) is a small double-stranded, circular DNA tumor virus encoding three tumor antigens: large T, Small T, and 17KT. These antigens contribute to cellular transformation, an area of research where they are utilized extensively. Transformation of cells by SV40 large T antigen is mediated by its binding to the tumor suppressor proteins RB and p53 in the nucleus. Additional binding partners include p300, CBP, Cul7, IRS1, Bub1, Nbs1, and Fbw7, which also contribute to transformation (1). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
Confocal immunofluorescent analysis of 293T (positive, left) and HeLa (negative, right) cells, using SV40 Large T antigen (D1E9E) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). | |
Western blot analysis of extracts from 293 and 293T cells using SV40 Large T Antigen (D1E9E) Rabbit mAb (upper) and β-Tubulin (D2N5G) Rabbit mAb #15115 (lower). |
