| 货号 | 5461S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-F |
| 目标/特异性 | Synaptophysin (D35E4) XP® Rabbit mAb detects endogenous levels of total synaptophysin protein. Some nuclear background staining was observed by confocal immunofluorescent analysis. |
| 使用方法 | WB(1:1000) IP (1:25) IF-F (1:100) |
| 供应商 | CST |
| 背景 | Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms, and thus can be used as a marker in tumor diagnosis (1). Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore (2). Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases (3). Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex (4). |
| 存放说明 | -20C |
| 计算分子量 | 38 |
Confocal immunofluorescent image of mouse brain labeled with Synaptophysin (D35E4) XP® Rabbit mAb (green) and β3-Tubulin (TU-20) Mouse mAb #4466 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析小鼠大脑，使用的抗体是Synaptophysin (D35E4) XP®Rabbit mAb 兔单抗（绿色）和β3-Tubulin (TU-20) Mouse mAb 鼠单抗 #4466（红色）。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。 | |
Western blot analysis of extracts from mouse and rat brain using Synaptophysin (D35E4) XP® Rabbit mAb. Western blot分析小鼠和大鼠大脑提取物，使用的抗体是Synaptophysin (D35E4) XP® Rabbit mAb 兔单抗。 |
