| 货号 | 4019S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Mouse |
| 应用 | W/IHC-P/IF-P/IF-F |
| 目标/特异性 | Tau (Tau46) Mouse mAb detects endogenous levels of total tau protein and also cross-reacts with MAP2 at 280kD. Tau (Tau46) Mouse mAb is predicted to detect all six isoforms of tau based on the amino acid sequence. |
| 使用方法 | WB(1:1000) IHC-P (1:400) IF-F (1:6400) IF-P (1:800) |
| 供应商 | CST |
| 背景 | Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy-terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by ERK, GSK-3 and CDK5 (1-2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimers disease and these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation of Ser396 by GSK-3 or CDK5 destabilizes microtubules in Alzheimers disease. Furthermore, inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3). |
| 存放说明 | -20C |
| 计算分子量 | 50 to 80 |
Immunohistochemical analysis of paraffin-embedded Alzheimers brain, using Tau (Tau46) Mouse mAb. 免疫组化分析石蜡包埋的阿尔茨海默病人大脑，使用的抗体是Tau (Tau46)Mouse mAb 鼠单抗。 | |
Western blot analysis of extracts from mouse and rat brain, using Tau (Tau46) Mouse mAb. Western blot分析小鼠和大鼠大脑，使用的抗体是Tau(Tau46) Mouse mAb 鼠单抗。 | |
Confocal immunofluorescent analysis of human Alzheimer brain (A) labeled with Tau (Tau46) Mouse mAb (green) and APP Antibody #2452 (red) compared to normal human brain (B). 共聚焦免疫荧光分析人阿尔茨海默病人大脑（A），使用抗体Tau (Tau46)Mouse mAb 鼠单抗（绿）和APP Antibody 兔多抗#2452（红）进行标记，并与正常人大脑（B）进行对照。 | |
Confocal immunofluorescent analysis of mouse dentate gyrus stained with Tau (Tau46) Mouse mAb (red) and CREB (86B10) Mouse mAb #9104 (blue). |
