| 货号 | 59710S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IF-IC |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Topoisomerases are ubiquitous, conserved enzymes that remove DNA supercoils resulting from processes such as chromosome segregation, DNA replication, transcription, and repair (1). Topoisomerase inhibitors such as camptothecin and etoposide trap the enzyme as a DNA-bound intermediate, and these drugs are used to treat multiple human cancers (1,2). Tyrosyl-DNA-phosphodiesterases TDP1 and TDP2 function in the base excision repair (BER) and nonhomologous end joining (NHEJ) DNA repair pathways, respectively, and function in part in the repair of stalled topoisomerase-DNA complexes (3). Research has shown that inhibitors of tyrosyl-DNA-phosphodiesterases may act synergistically with topoisomerase inhibitors, allowing the potential for a more robust treatment of cancer (4,5). In small cell lung cancer, research suggests that TDP1 and topoisomerase 1 levels can predict sensitivity to topoisomerase 1 inhibitors (6). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
Confocal immunofluorescent analysis of MDA-MB-231 (left, high expressing), SK-MEL-28 (center, moderately expressing), and MRC-5 (right, lower expressing) cells using TDP1 (D8D1B) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). | |
Western blot analysis of extracts from various cell lines using TDP1 (D8D1B) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). |
