| 货号 | 14971S |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IHC-P/F |
| 使用方法 | WB(1:1000) IHC-P (1:100) |
| 供应商 | CST |
| 背景 | T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-1 (HAVCR1 (hepatitis A virus cellular receptor 1), KIM-1 (kidney injury molecule-1) was originally identified as a receptor for hepatitis A virus (1). TIM-1 also acts as a costimulatory receptor on T cells and following activation, associates with the TCR complex to upregulate signaling and cytokine production (2-5). Another TIM family member, TIM-4, is expressed by antigen presenting cells and is a ligand for TIM-1 (6). TIM-1 expressed by Th1 and Th17 cells was also recently shown to interact with P-selectin to mediate T cell trafficking during inflammation and autoimmune disease (7). NKT cells also express TIM-1, and engagement of TIM-1 on NKT cells leads to increased production of IL-4, but decreased production of IFN-gamma (8). TIM-1 is also a receptor for phosphatidylserine exposed by cells undergoing apoptosis. Detection of phosphatidylserine by TIM-1 expressed on NKT cells results in activation, proliferation, and cytokine production (9). Expression of TIM-1 on regulatory B cells is required for optimal production of IL-10. Mice lacking the TIM-1 mucin domain have decreased production of IL-10 by regulatory B cells, hyperactive T cells, increased levels of inflammatory cytokines, and enhanced severity of autoimmune disease (10,11). In addition, TIM-1 polymorphisms are associated with susceptibility to atopic diseases including asthma (12,13). Finally, expression of TIM-1 is increased in renal tubular epithelial cells following kidney injury (14). |
| 存放说明 | -20C |
| 计算分子量 | 50, 90-140 |
Immunohistochemical analysis of paraffin-embedded normal human kidney using TIM-1 (E1R9N) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded ACHN (left) and HeLa (right) cell pellets using TIM-1 (E1R9N) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using TIM-1 (E1R9N) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human papillary renal cell carcinoma using TIM-1 (E1R9N) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Western blot analysis of extracts from various cell lines using TIM-1 (E1R9N) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full-length human TIM-1 (hTIM-1-DDK, +), using TIM-1 (E1R9N) Rabbit mAb. |
