| 货号 | 4746S |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | TRA-1-60(S) (TRA-1-60(S)) Mouse mAb detects endogenous levels of the neuraminidase-sensitive TRA-1-60 antigen. |
| 使用方法 | WB(1:1000) IHC-P (1:100) F (1:400) IF-IC (1:1000) |
| 供应商 | CST |
| 背景 | TRA-1-60 and TRA-1-81 antibodies detect antigens present on the surface of human stem, teratocarcinoma, and embryonic germ cells (1). TRA-1-60(S) reacts with a neuraminidase sensitive epitope of a proteoglycan (2,3), while TRA-1-81 reacts with a neuraminidase insensitive epitope on the same antigen. Recently this antigen has been proposed to be a form of the protein podocalyxin (4). TRA-1-60 is also detected in the serum of patients with germ cell tumors (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 200-400 |
Confocal immunofluorescent analysis of nTERA2 cells using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用TRA-1-60(S) (TRA-1-60(S)) 鼠源单克隆抗体（绿色）对nTERA2细胞进行激光共聚焦荧光分析实验。使用DY-554鬼笔环肽（红色）标记肌动蛋白丝。蓝色假色=DRAQ5® #4084 (荧光DNA染料). | |
Western blot analysis of extracts from various cell lines using TRA-1-60 (TRA-1-60) Mouse mAb.使用TRA-1-60（TRA-1-60）鼠源单克隆抗体对多种细胞提取物进行Western blot实验分析。 | |
Flow cytometric analysis of unpermeabilized Jurkat cells (blue) and unpermeabilized NTERA2 cells (green) using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb.使用TRA-1-60(S) (TRA-1-60(S))鼠源单克隆抗体对不可透化的Jurkat 细胞（蓝色）和不可透化的NTERA2细胞（绿色）进行流式细胞分析。 | |
Immunohistochemical analysis of paraffin-embedded NTERA (positive, left) and Jurkat (negative, right) cell pellets using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb.使用TRA-1-60(S) (TRA-1-60(S))鼠源单克隆抗体对石蜡包埋的NTERA（阳性，左）和Jurkat（阴性，右）进行免疫组化分析。 |
