| 货号 | 4745S |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | IHC-P/IF-IC/F |
| 目标/特异性 | TRA-1-81 antibody detects endogenous levels of TRA-1-81 antigen. |
| 使用方法 | IHC-P(1:100) F (1:200) IF-IC (1:250) |
| 供应商 | CST |
| 背景 | TRA-1-60 and TRA-1-81 antibodies detect antigens present on the surface of human stem, teratocarcinoma, and embryonic germ cells (1). TRA-1-60(S) reacts with a neuraminidase sensitive epitope of a proteoglycan (2,3), while TRA-1-81 reacts with a neuraminidase insensitive epitope on the same antigen. Recently this antigen has been proposed to be a form of the protein podocalyxin (4). TRA-1-60 is also detected in the serum of patients with germ cell tumors (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 200-400 |
Confocal immunofluorescent analysis of NTERA-2 cells using TRA-1-81 (TRA-1-81) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用TRA-1-81 (TRA-1-81) 鼠源单克隆抗体（绿色）对NTERA-2细胞进行激光共聚焦荧光分析。用DY-554鬼笔环肽（红色）标记肌动蛋白丝。蓝色假色=DRAQ5® #4084 (荧光DNA染料). | |
Flow cytometric analysis of unpermeabilized Jurkat cells (blue) and unpermeabilized NCCIT cells (green) using TRA-1-81 (TRA-1-81) Mouse mAb.用TRA-1-81 (TRA-1-81) 鼠源单克隆抗体对不可透化的Jurkat 细胞（蓝色）和不可透化的NCCIT细胞（绿色）进行流式细胞分析。 | |
Immunohistochemical analysis of paraffin-embedded NTERA (positive, left) and Jurkat (negative, right) cell pellets using TRA-1-81 (TRA-1-81) Mouse mAb.使用TRA-1-81 (TRA-1-81) 鼠源单克隆抗体对石蜡包埋的NTERA（阳性，左）和Jurkat（阴性，右）进行免疫组化分析。 |
