| 货号 | 3376S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC |
| 目标/特异性 | TrkC (C44H5) Rabbit mAb detects endogenous levels of total TrkC protein. The antibody does not cross-react with TrkA or TrkB. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:2000) IF-IC (1:1600) |
| 供应商 | CST |
| 背景 | The family of Trk receptor tyrosine kinases consists of TrkA, TrkB and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3. TrkA regulates proliferation and is important for development and maturation of the nervous system (1). Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at this site reflects TrkA kinase activity (2-6). Point mutations, deletions and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA. Many malignancies including breast, colon, prostate and thyroid carcinomas and acute myeloid leukemia have activated TrkA. Expression of TrkA in neuroblastomas is a good prognostic marker because it signals growth arrest and differentiation of cells originating from the neural crest (1).The phosphorylation sites are conserved between TrkA and TrkC: Tyr490 of TrkA corresponds to Tyr516 in TrkC, and Tyr674/675 of TrkA to Tyr709/710 in TrkC of the human sequence (7). Altered TrkC expression and corresponding gene mutations are seen in various forms of cancer, with increased expression a positive prognostic indicator in patients with medulloblastoma (8). |
| 存放说明 | -20C |
| 计算分子量 | 145, 100 |
Western blot analysis of extracts from mouse and rat brain using TrkC (C44H5) Rabbit mAb. Western blot分析小鼠和大鼠大脑提取物，使用的抗体是TrkC (C44H5)Rabbit mAb 兔单抗。 | |
Confocal immunofluorescent analysis of NIH/3T3 cells, wild-type (upper left), NIH/3T3/TrkA (upper right), NIH/3T3/TrkB (lower left) or NIH/3T3/TrkC (lower right) using TrkC (C44H5) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析NIH/3T3细胞，野生型(上左)、NIH/3T3/TrkA（上右）、NIH/3T3/TrkB（下左）或NIH/3T3/TrkC（下右），使用的抗体是TrkB (C44H5)Rabbit mAb兔单抗（绿色）。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。 | |
Immunohistochemical analysis of paraffin-embedded rat brain using TrkC (C44H5) Rabbit mAb (left) compared to concentration matched Rabbit (DA1E) mAb IgG XP(R) Isotype Control #3900 (right). | |
免疫组化分析石蜡包埋份大鼠大脑，使用的抗体是TrkC (C44H5) Rabbit mAb兔单抗 (左图) ，与之比较的是 Rabbit (DA1E) mAb IgG XP(R) Isotype Control #3900 (右图)。 |
