| 货号 | 3936S |
| 反应种属 | All |
| 来源宿主 | Mouse |
| 应用 | W/IHC-P |
| 目标/特异性 | Ubiquitin (P4D1) Mouse mAb detects ubiquitin, polyubiquitin and ubiquitinated proteins. This antibody may cross-react with recombinant NEDD8.Species Reactivity: All Species Expected. |
| 使用方法 | WB(1:1000) IHC-P (1:375) |
| 供应商 | CST |
| 背景 | Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as IκB, p53, cdc25A, and Bcl-2 have been shown to be targets for the ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis (4-7). |
| 存放说明 | -20C |
Western blot analysis of 293 and HeLa cells, untreated or treated with the 26S proteasome inhibitor MG132 (50 µM, 90 minutes), using Ubiquitin (P4D1) Mouse mAb. | |
使用泛素（P4D1）小鼠单克隆抗体对未经或经26S蛋白酶体抑制剂MG132处理(50 µM，90分钟)的293和HeLa细胞裂解物的免疫印迹分析结果。 |
