| 货号 | 2708S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | p70 S6 Kinase (49D7) Rabbit mAb detects endogenous levels of total p70 S6 kinase protein. The antibody also recognizes p85 S6 kinase. |
| 使用方法 | WB(1:1000) IHC-P (1:480) |
| 供应商 | CST |
| 背景 | p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1,2). p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5 oligopyrimidine tract mRNAs (1). A second isoform, p85 S6 kinase, is derived from the same gene and is identical to p70 S6 kinase except for 23 extra residues at the amino terminus, which encode a nuclear localizing signal (1). Both isoforms lie on a mitogen activated signaling pathway downstream of phosphoinositide-3 kinase (PI-3K) and the target of rapamycin, FRAP/mTOR, a pathway distinct from the Ras/MAP kinase cascade (1). The activity of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains (1). Phosphorylation of Thr229 in the catalytic domain and Thr389 in the linker domain are most critical for kinase function (1). Phosphorylation of Thr389, however, most closely correlates with p70 kinase activity in vivo (3). Prior phosphorylation of Thr389 is required for the action of phosphoinositide 3-dependent protein kinase 1 (PDK1) on Thr229 (4,5). Phosphorylation of this site is stimulated by growth factors such as insulin, EGF and FGF, as well as by serum and some G-protein-coupled receptor ligands, and is blocked by wortmannin, LY294002 (PI-3K inhibitor) and rapamycin (FRAP/mTOR inhibitor) (1,6,7). Ser411, Thr421 and Ser424 lie within a Ser-Pro-rich region located in the pseudosubstrate region (1). Phosphorylation at these sites is thought to activate p70 S6 kinase via relief of pseudosubstrate suppression (1,2). Another LY294002 and rapamycin sensitive phosphorylation site, Ser371, is an in vitro substrate for mTOR and correlates well with the activity of a partially rapamycin resistant mutant p70 S6 kinase (8). |
| 存放说明 | -20C |
| 计算分子量 | 70, 85 |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing cytoplasmic and nuclear localization, using p70 S6 Kinase (49D7) Rabbit mAb. 免疫组织化学方法检测石蜡包埋的人肺癌组织，显示胞质和核定位，使用的抗体是p70 S6 Kinase (49D7) Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using p70 S6 Kinase (49D7) Rabbit mAb in the presence of control peptide (left) or p70 S6 Kinase Blocking Peptide #1205 (right). 免疫组织化学方法检测石蜡包埋的人乳腺癌组织，分别用对照肽段（左图）和p70 S6 Kinase Blocking Peptide #1205 （右图）处理，使用的抗体是p70 S6 Kinase (49D7) Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using p70 S6 Kinase (49D7) Rabbit mAb. 免疫组织化学方法检测石蜡包埋的人结肠癌组织，使用的抗体是p70 S6 Kinase (49D7) Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from PC12, NIH/3T3, and SK-N-MC cells, using p70 S6 Kinase (49D7) Rabbit mAb. Western blot 方法检测细胞提取物：PC12, NIH/3T3和 SK-N-MC细胞，使用的抗体是p70 S6 Kinase (49D7) Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® p70/85 S6 Kinase siRNA I (+) or SignalSilence® p70/85 S6 Kinase siRNA II #6572 (+), using p70 S6 Kinase (49D7) Rabbit mAb #2708 and α-Tubulin (11H10) Rabbit mAb #2125. The p70 S6 Kinase (49D7) Rabbit mAb confirms silencing of p70/85 S6 kinase expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of p70/85 S6 kinase siRNA. Western blot方法检测Hela细胞提取物：分别用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® p70/85 S6 Kinase siRNA I (+) 和 SignalSilence® p70/85 S6 Kinase siRNA II #6572 (+)转染，使用抗体是 p70 S6 Kinase (49D7) Rabbit mAb #2708 和 α-Tubulin (11H10) Rabbit mAb 兔单抗#2125。 p70 S6 Kinase (49D7) Rabbit mAb兔单抗可以确认 p70/85 S6 kinase 是否沉默，而 α-Tubulin (11H10) Rabbit mAb兔单抗作为上样量和 p70/85 S6 kinase siRNA特异性的对照。 |
