| 货号 | 9411S |
| 反应种属 | Human/Mouse/Rat/All |
| 来源宿主 | Mouse |
| 应用 | W/IP/IHC-P/IF-P/IF-F/IF-IC/F/E-P |
| 目标/特异性 | Phospho-Tyrosine Mouse mAb (P-Tyr-100) is a high affinity antibody. ELISAs against a wide variety of phosphopeptides indicate that (a) P-Tyr-100 binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequence. 2D gel Western blot analysis of pervanadate-treated cell extracts also shows that P-Tyr-100 interacts with a broad range of tyrosine-phosphorylated proteins. P-Tyr-100 does not cross-react with peptides containing phospho-Ser or phospho-Thr. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | WB(1:2000) IP (1:100) IHC-P (1:4800) F (1:3200) IF-F (1:1600) IF-IC (1:1600) IF-P (1:1600) E-P (1:4000) |
| 供应商 | CST |
| 背景 | Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. |
| 存放说明 | -20C |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Tyrosine Mouse mAb (P-Tyr-100). 对石蜡包埋的人乳腺癌使用Phospho-Tyrosine Mouse mAb (P-Tyr-100)进行免疫组化分析。 | |
Immunofluorescent analysis of Swiss NIH/3T3 cells, serum-starved and stimulated with lysophosphatidic acid (LPA) (10 µM for 10 minutes) and fixed with PFA, using Phospho-Tyrosine Mouse mAb (P-Tyr-100) (red) and phalloidin for F-actin (green) LPA causes heavy tyrosine phosphorylation of proteins in focal adhesions, present at the tips of actin stress fibers. (Provided by Dr. Harry Mellor, University of Bristol.) 对Swiss NIH/3T3细胞，血清饥饿和溶血磷脂酸（LPA）10 uM处理10分钟后用PFA固定，使用Phospho-Tyrosine Mouse mAb (P-Tyr-100)（红），phalloidin用于标记F-actin（绿）LPA导致局部粘合蛋白的酪氨酸磷酸化出现在肌动蛋白纤维尖端。（Dr. Harry Mellor, University of Bristol提供） | |
Western blot analysis of extracts from Jurkat cells treated with 1 mM pervanadate for 30 minutes prior to lysis, using P-Tyr-100 Phospho-Tyrosine Mouse mAb. Proteins were separated by 2-D electrophoresis prior to blotting. 对1mM过钒酸盐裂解前处理30分钟的Jurkat细胞抽提液使用P-Tyr-100 Phospho-Tyrosine Mouse mAb进行Western blot分析。蛋白质在印记前在二维电泳分离。 | |
Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate) ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (Y* denotes phosphorylated tyrosine.) Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate) ELISA分析：磷酸化和非磷酸化的信噪比。（Y* 代表磷酸化的酪氨酸） | |
Flow cytometric analysis of K562 cells, untreated (green) or Gleevec®- treated (blue), using Phospho-Tyrosine Mouse mAb (P-Tyr-100) compared to a nonspecific negative control antibody (red). 对K562细胞，未处理（绿）或Gleevec®处理（蓝），使用Phospho-Tyrosine Mouse mAb (P-Tyr-100)和非特异性阴性对照抗体（红色），进行流式细胞仪分析。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-Tyrosine Mouse mAb (P-Tyr-100). 对石蜡包埋的人肺癌使用Phospho-Tyrosine Mouse mAb (P-Tyr-100)进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded NCI-H1650 xenograft untreated (left) or lambda-phosphatase-treated (right), using Phospho-Tyrosine Mouse mAb (P-Tyr-100). 对石蜡包埋的NCI-H1650移植物，未处理（左）或λ磷酸酶处理（右），使用Phospho-Tyrosine Mouse mAb (P-Tyr-100)进行免疫组化分析。 | |
Confocal immunofluorescence analysis of HeLa cells either stimulated with 20% serum (left) or untreated (right), using Phospho-Tyrosine Mouse mAb (P-Tyr-100) (red). Actin filaments have been labeled with fluorescein phalloidin. Blue pseudocolor = DRAQ5® (fluorescent DNA dye). 对HeLa细胞，20%血清刺激（左）或未处理（右），使用Phospho-Tyrosine Mouse mAb (P-Tyr-100)（红）进行免疫组化分析。肌动蛋白用fluorescein phalloidin标记。蓝色伪彩=DRAQ5®(荧光DNA染料)。 | |
Confocal immunofluorescent analysis of paraffin-embedded human lung adenocarcinoma using Phospho-Tyrosine Mouse mAb (P-Tyr-100) (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye). 对石蜡包埋的人肺腺癌使用Phospho-Tyrosine Mouse mAb (P-Tyr-100)（红）进行共聚焦免疫荧光分析。蓝色伪彩=DRAQ5®(荧光DNA染料)。 |
