| 货号 | 13175T |
| 同种亚型 | Rabbit |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | Phospho-PRAS40 (Thr246) (D4D2) XP® Rabbit mAb recognizes endogenous levels of PRAS40 protein only when phosphorylated at Thr246. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt (1,2). One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 proteins (2). PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity (3). PRAS40 interacts with raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1 (4). mTORC1 in turn phosphorylates PRAS40 at Ser183 (5). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
| 参考文献 | 1 . Cantley, L.C. (2002) Science 296, 1655-7. 2 . Kovacina, K.S. et al. (2003) J Biol Chem 278, 10189-94. 3 . Vander Haar, E. et al. (2007) Nat Cell Biol 9, 316-23. 4 . Sancak, Y. et al. (2007) Mol Cell 25, 903-15. 5 . Oshiro, N. et al. (2007) J Biol Chem 282, 20329-39. |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-PRAS40 (Thr246) (D4D2) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Immunohistochemical analysis of paraffin-embedded metastatic SK-OV-3 tumor in mouse lung using Phospho-PRAS40 (Thr246) (D4D2) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right) using Phospho-PRAS40 (Thr246) (D4D2) XP® Rabbit mAb. Cells used are from the SignalSlide® Phospho-Akt (Ser473) IHC Controls #8101. | |
Western blot analysis of extracts from serum starved HeLa cells, untreated (-), insulin-treated (1 μM, 30 min; +), or insulin and λ phosphatase-treated (+), using Phospho-PRAS40 (Thr246) (D4D2) XP® Rabbit mAb (upper) or PRAS40 Antibody #2610 (lower). |
