Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate)【科瑞奥博】

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Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate)

货号： 2975S 基本售价： 1672.0 元规格： 100ul

产品信息

概述
货号	2975S
描述	This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells.
反应种属	Human,
来源宿主	Rabbit IgG
应用	IF-IC/F
使用方法	IF-IC ()

性能
供应商	CST
标记	Alexa Fluor 488
背景	Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
存放说明	4C

参考图片

Flow cytometric analysis of Jurkat cells, using Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) (red) compared to Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #4071, untreated (green) or LY294002- and wortmannin-treated (blue).流式细胞术分析Jurkat细胞，所用抗体Rabbit (DA1E) mAb IgG XP ® 同型对照(Alexa Fluor ® 488 Conjugate) (红色) 与Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) #4071作比较, (绿色)为未处理组，蓝色为LY294002 和wortmannin处理组。

Flow cytometric analysis of Jurkat cells, using Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) (red) compared to Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #4803, untreated (blue) or IFN-α-treated (green).流式细胞术分析Jurkat细胞，所用抗体为Rabbit (DA1E) mAb IgG XP ®同型对照 Alexa Fluor ® 488 Conjugate) (红色) ，与Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) #4803,作比较, 蓝色为未处理组，绿色为IFN-α 处理组。

Confocal immunofluorescent analysis of Cos cells using GAPDH (14C10) Rabbit mAb (Alexa Fluor® 488 Conjugate) #3906 (green, left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate (green, right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光分析COS细胞，所用抗体为GAPDH (14C10) Rabbit mAb (Alexa Fluor ® 488 Conjugate) #3906 (绿色，左图) 与浓度匹配的Rabbit (DA1E) mAb IgG XP 同型对照 (Alexa Fluor ® 488 Conjugate (绿色，右图)作比较。蓝色伪彩为荧光染料，信息为DRAQ5 ® #4084。