IRF-3 (D6I4C) XP® Rabbit mAb【科瑞奥博】

产品中心

当前位置：首页>产品中心

IRF-3 (D6I4C) XP® Rabbit mAb

货号： 11904T 基本售价： 1550.0 元规格： 20ul

产品信息

概述
货号	11904T
同种亚型	Rabbit IgG
反应种属	Human/Monkey
来源宿主	Rabbit
应用	W/IP/IF-IC
目标/特异性	IRF-3 (D6I4C) XP® Rabbit mAb recognizes endogenous levels of total IRF-3 protein.
使用方法	WB(1:1000) IP (1:50) IF-IC (1:400)

性能
供应商	CST
灵敏度	Endogenous
背景	Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2).
存放说明	-20C
计算分子量	50-55
参考文献	1 . Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55. 2 . Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58. 3 . Hiscott, J. et al. (1999) J Interferon Cytokine Res 19, 1-13. 4 . Kim, T.Y. et al. (2003) J Biol Chem 278, 15272-8. 5 . Yoneyama, M. et al. (2002) J Interferon Cytokine Res 22, 73-6. 6 . Fitzgerald, K.A. et al. (2003) Nat Immunol 4, 491-6. 7 . Kopp, E. and Medzhitov, R. (2003) Curr Opin Immunol 15, 396-401. 8 . Servant, M.J. et al. (2003) J Biol Chem 278, 9441-7.

参考图片

Confocal immunofluorescent analysis of HT-29 cells, untransfected (left) or transfected with Poly(I:C) (2.5 μg/ml, 6 hr; right), using IRF-3 (D6I4C) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦分析HT-29细胞，未转染(左图)或转染Poly(I:C) (2.5 μg/ml, 6小时; 右图)，使用抗体是IRF-3 (D6I4C) XP® Rabbit mAb (绿色)。蓝色= DRAQ5® #4084 (DNA荧光染料)。

Immunoprecipitation of IRF-3 from THP-1 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or IRF-3 (D6I4C) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using IRF-3 (D6I4C) XP® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 and Anti-mouse IgG, HRP-linked Antibody #7076 were used as secondary antibodies.对THP-1细胞提取物中的IRF-3进行免疫沉淀，使用抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2) 或 IRF-3 (D6I4C) XP® Rabbit mAb (泳道3)。泳道1为10%的加入量。Western blot分析使用的抗体是IRF-3 (D6I4C) XP® Rabbit mAb。Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 和 Anti-mouse IgG, HRP-linked Antibody #7076用来作为二抗。

Western blot analysis of extracts from various cell lines using IRF-3 (D6I4C) XP® Rabbit mAb.Western blot分析多种细胞系的细胞提取物，使用抗体是IRF-3 (D6I4C) XP® Rabbit mAb。