| 货号 | 12480T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | GM130 (D6B1) XP® Rabbit mAb recognizes endogenous levels of total GM130 protein. This antibody may cross-react with a protein of unknown origin at 30 kDa. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:3200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Golgi apparatus functions in the modification, organization, and transport of proteins and membranes targeted to other parts of the cell, such as the plasma membrane, lysosomes, and endosomes. This regulated transport is important for appropriate protein localization, secretion, and signal transduction. Members of the Golgin family of proteins, including GM130, Giantin, p115, and GRASP65, are defined by their presence in the Golgi matrix and by their long coiled coil domains. Golgin function, which is regulated in part by small GTPases of the Rab and Arl families, includes establishing and maintaining Golgi structure and transport (reviewed in 1). The Golgi cisternae are stacked and linked laterally to form a ribbon. GRASP65 and GM130 are required for membrane fusion events that mediate ribbon formation during Golgi assembly. These lateral fusion events allow for uniform distribution of Golgi enzymes (2). GM130 and Giantin interact with the transport factor p115 to facilitate endoplasmic reticulum (ER)-Golgi transport (3). GM130 is also involved in the transport of the Ether-a-go-go-Related (hERG) potassium ion channel. Inappropriate hERG localization may be an underlying cause in Long QT syndrome, a hereditary and potentially fatal cardiac arrhythmia (4). Further, GM130 was implicated in signal transduction regulating invasion, migration, and cell polarization via its interaction with and activation of serine/threonine kinases YSK1 and Mst4 (5). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
| 参考文献 | 1 . Barr, F.A. and Short, B. (2003) Curr Opin Cell Biol 15, 405-13. 2 . Puthenveedu, M.A. et al. (2006) Nat Cell Biol 8, 238-48. 3 . Alvarez, C. et al. (2001) J. Biol. Chem. 276, 2693-2700. 4 . Roti, E.C. et al. (2002) J. Biol. Chem. 277, 47779-47785. 5 . Preisinger, C. et al. (2004) J. Cell Biol. 164, 1009-1020. |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Brefeldin A #9972 (200 μM, 30 min; right), using GM130 (D6B1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).未处理（左）或使用Brefeldin A #9972 (200 μM, 30 min;右)处理HeLa细胞获得细胞提取物，使用GM130 (D6B1) XP® Rabbit mAb（绿色）进行激光共聚焦免疫荧光分析。肌动蛋白丝使用DY-554鬼笔环肽标记（红色）。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using GM130 (D6B1) XP® Rabbit mAb. 使用GM130 (D6B1) XP® Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Immunoprecipitation of GM130 protein from ZR-75-1 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or GM130 (D6B1) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using GM130 (D6B1) XP® Rabbit mAb.使用 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或GM130 (D6B1) XP® Rabbit mAb (lane 3)对ZR-75-1细胞提取物的GM130进行免疫沉淀实验。Lane1是10%上样量。使用GM130 (D6B1) XP® Rabbit mAb进行western blot分析。 |
