| 货号 | 13856T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC |
| 目标/特异性 | PGRMC1 (D6M5M) XP® Rabbit mAb recognizes endogenous levels of total PGRMC1 protein. This antibody does not cross-react with PGRMC2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The progesterone receptor membrane component 1 (PGRMC1, Hpr6.6) was originally identified as a component of a progesterone-binding protein complex that also contains plasminogen activator inhibitor 1 RNA binding protein (PAIRBP1, SERBP1) (1,2). The structure of PGRMC1 protein includes a single transmembrane region and a carboxy-terminal cytochrome b5 heme-binding domain (3,4). Research studies confirm that PGRMC1 binds heme as well as binding and regulating cytochrome P450 enzymes responsible for the metabolism of clinical drugs and endogenous signaling molecules (5-7). While early research studies were equivocal on the ability of PGRMC1 to bind progesterone, studies using PGRMC1-fusion proteins clearly demonstrate that PGRMC1 binds progesterone with high affinity (2,8). Studies detailing expression of PGRMC1 in granulosa cells suggest that PGRMC1 mediates the anti-apoptotic actions of progesterone and that this protein is part of a signal transduction pathway that regulates granulosa cell function (9). |
| 存放说明 | -20C |
| 计算分子量 | 25 |
| 参考文献 | 1 . Cahill, M.A. (2007) J Steroid Biochem Mol Biol 105, 16-36. 2 . Peluso, J.J. et al. (2008) Endocrinology 149, 534-43. 3 . Gerdes, D. et al. (1998) Biol Chem 379, 907-11. 4 . Mifsud, W. and Bateman, A. (2002) Genome Biol 3, RESEARCH0068. 5 . Crudden, G. et al. (2006) J Pharmacol Exp Ther 316, 448-55. 6 . Hughes, A.L. et al. (2007) Cell Metab 5, 143-9. 7 . Oda, S. et al. (2011) Drug Metab Dispos 39, 2057-65. 8 . Peluso, J.J. et al. (2009) J Clin Endocrinol Metab 94, 2644-9. 9 . Peluso, J.J. (2013) Front Neurosci 7, 99. |
Confocal immunofluorescent analysis of 293T cells using PGRMC1 (D6M5M) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma using PGRMC1 (D6M5M) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung non-small cell carcinoma using PGRMC1 (D6M5M) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Western blot analysis of extracts from various cell lines using PGRMC1 (D6M5M) XP® Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human PGRMC1 protein (hPGRMC1-Myc; +), using PGRMC1 (D6M5M) XP® Rabbit mAb (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower). | |
Immunoprecipitation of PGRMC1 from MCF7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PGRMC1 (D6M5M) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PGRMC1 (D6M5M) XP® Rabbit mAb. |
