| 货号 | 14896T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | MIST1/bHLHa15 (D7N4B) XP®Rabbit mAb recognizes endogenous levels of total MIST1/bHLHa15 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Class A basic helix-loop-helix protein 15 (MIST1, bHLHa15) is a highly conserved basic helix loop helix family transcription factor that binds E-box motifs and regulates the expression of developmentally regulated genes (1). MIST1 can bind DNA as a homodimer, or may heterodimerize with other bHLH proteins to regulate target gene expression (1). MIST1 is expressed in an array of tissues, including salivary glands, stomach, small intestine, and the pancreas, but is generally restricted to secretory cell subtypes (2). In the pancreas, MIST1 is essential for the maturation, maintenance, and function of acinar cells (3). In gastric chief cells, MIST1 regulates the expression of RAB26 and RAB3D, two GTPases that function to regulate secretory granule formation (4). Loss of MIST1 in gastric chief cells may be a potential marker of gastric epithelial neoplasia (5). |
| 存放说明 | -20C |
| 计算分子量 | 23 |
| 参考文献 | 1 . Lemercier, C. et al. (1997) Dev Biol 182, 101-13. 2 . Pin, C.L. et al. (2000) Anat Rec 259, 157-67. 3 . Pin, C.L. et al. (2001) J Cell Biol 155, 519-30. 4 . Tian, X. et al. (2010) Mol Cell Biol 30, 1269-84. 5 . Lennerz, J.K. et al. (2010) Am J Pathol 177, 1514-33. |
Western blot analysis of extracts from human pancreas, mouse pancreas, and human colon using MIST1/bHLHa15 (D7N4B) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunohistochemical analysis of paraffin-embedded human pancreas using MIST1/bHLHa15 (D7N4B) XP®Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse pancreas using MIST1/bHLHa15 (D7N4B) XP®Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse spleen using MIST1/bHLHa15 (D7N4B) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse small intestine using MIST1/bHLHa15 (D7N4B) XP®Rabbit mAb. |
