Anti-Cytochrome C【科瑞奥博】

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Anti-Cytochrome C

货号： bs-0013R 基本售价： 380.0 元规格： 20ul

规格：20ul: 价格：380.00元

规格：50ul: 价格：780.00元

规格：100ul: 价格：1380.00元

规格：200ul: 价格：2200.00元

产品信息

产品编号: bs-0013R

英文名称: Cytochrome C

中文名称: 细胞色素C抗体

别名: CytC; CYC; CYCS; Cytochrome c somatic; HCS; CYC_HUMAN; Cytochrome c; MSA06; THC4.

: Specific References  (14)     |     bs-0013R has been referenced in 14 publications.
[IF=2.94] Wang, Shuhua, et al. "μ-Calpain mediates hippocampal neuron death in rats after lithium–pilocarpine-induced status epilepticus." Brain research bulletin 76.1 (2008): 90-96.  WB ;  Rat.
PubMed:18395616
[IF=3.12] Jiang, H-Q., et al. "Guanabenz Delays the Onset of Disease Symptoms, Extends Lifespan, Improves Motor Performance and Attenuates Motor Neuron Loss in the SOD1 G93A Mouse Model of Amyotrophic Lateral Sclerosis." Neuroscience (2014).  WB ;  Mouse.
PubMed:24699224
[IF=2.55] Chen, Chengzhi, et al. "Resveratrol protects against arsenic trioxide‐induced oxidative damage through maintenance of glutathione homeostasis and inhibition of apoptotic progression." Environmental and Molecular Mutagenesis (2014).  WB ;  Human.
PubMed:25339131
[IF=1.61] Gu, Shiyan, et al. "Resveratrol Synergistically Triggers Apoptotic Cell Death with Arsenic Trioxide via Oxidative Stress in Human Lung Adenocarcinoma A549 Cells." Biological trace element research (2014): 1-12.  WB ;  Human.
PubMed:25431299
[IF=3.53] Fang C, Zhang J, Qi D, Fan X, Luo J, et al. (2014) Evodiamine Induces G2/M Arrest and Apoptosis via Mitochondrial and Endoplasmic Reticulum Pathways in H446 and H1688 Human Small-Cell Lung Cancer Cells. PLoS ONE 9(12): e115204.   WB ;  Human.
PubMed:25506932
[IF=1.48] Gao, Guanmin, et al. "Poly (ADP‑ribose) polymerase‑and cytochrome c‑mediated apoptosis induces hepatocyte injury in a rat model of hyperammonia‑induced hepatic failure." Molecular Medicine Reports.  WB ;  Rat.
PubMed:25634059
[IF=1.71] Liao, Peng, et al. "Organellar proteome analyses of ricin toxin-treated HeLa cells." Toxicology and industrial health (2014): 0748233714549066.  WB ;  Human.
PubMed:25227225
[IF=2.59] Li, Aihong, et al. "Increased Expression of Mitochondrial Inner-Membrane Protein Mpv17 After Intracerebral Hemorrhage in Adult Rats." Neurochemical Research (2015): 1-11.  WB ;  Rat.
PubMed:26123482
[IF=3.20] Chen, Chengzhi, et al. "Nuclear translocation of nuclear factor kappa B is regulated by G protein signaling pathway in arsenite‐induced apoptosis in HBE cell line." Environmental Toxicology (2015).  WB ;  Human.
PubMed:26306706
[IF=2.58] Gu, Shiyan, et al. "ROS-mediated endoplasmic reticulum stress and mitochondrial dysfunction underlie apoptosis induced by resveratrol and arsenic trioxide in A549 cells." Chemico-Biological Interactions (2016).  WB ;  Human.
PubMed:26772155
[IF=5.74] Duan, Xiaoxu, et al. "Antioxidant tert-butylhydroquinone ameliorates arsenic-induced intracellular damages and apoptosis through induction of Nrf2-dependent antioxidant responses as well as stabilization of anti-apoptotic factor Bcl− 2 in human keratinocytes." Free Radical Biology and Medicine(2016).  WB ;  Human.
PubMed:26878773
[IF=1.93] Ye, Jinxia, et al. "Millimeter Wave Treatment Inhibits Apoptosis of Chondrocytes via Regulation Dynamic Equilibrium of Intracellular Free Ca2."Evidence-Based Complementary and Alternative Medicine 2015 (2015).  Rat.
PubMed:25705239
[IF=2.57] Liu, Yi, et al. "GluR3B Ab’s induced oligodendrocyte precursor cells excitotoxicity via mitochondrial dysfunction." Brain Research Bulletin (2017).  IF(ICC) ;  Rat.
PubMed:28063880
[IF=2.38] Abdel-Hamid, Nagwa I., Mona F. El-Azab, and Yasser M. Moustafa. "Macrolide antibiotics differentially influence human HepG2 cytotoxicity and modulate intrinsic/extrinsic apoptotic pathways in rat hepatocellular carcinoma model." Naunyn-Schmiedebergs Archives of Pharmacology (2017): 1-17.  IHC-P ;  Rat.
PubMed:28070612

规格价格: 50ul/780元购买 100ul/1380元购买 200ul/2200元购买大包装/询价

说明书: 50ul 100ul 200ul

研究领域: 肿瘤心血管细胞生物神经生物学信号转导细胞凋亡脂蛋白线粒体

抗体来源: Rabbit

克隆类型: Polyclonal

交叉反应: Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, Guinea Pig,

产品应用: WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分子量: 12.8/26kDa

细胞定位: 细胞浆细胞膜线粒体

性状: Lyophilized or Liquid

浓度: 1mg/ml

免疫原: KLH conjugated synthetic peptide derived from human Cytochrome C:51-105/105

亚型: IgG

纯化方法: affinity purified by Protein A

储存液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed: PubMed

产品介绍

background:
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP. An increasing amount of interest has been directed toward the role which cytocrome C has been demonstrated to play in apoptotic processes. Following exposure to apoptotic stimuli, cytochrome C is rapidly released from the mitochondria into the cytosol, an event which may be required for the completion of apoptosis in some systems. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

Function:
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

Subcellular Location:
Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane.

Post-translational modifications:
Binds 1 heme group per subunit.
Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.

DISEASE:
Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.

Similarity:
Belongs to the cytochrome c family.

SWISS:
P99999

Gene ID:
54205

Database links:

Entrez Gene: 54205 Human

Entrez Gene: 13063 Mouse

Entrez Gene: 25309 Rat

Omim: 123970 Human

SwissProt: P99999 Human

SwissProt: P62897 Mouse

SwissProt: P62898 Rat

Unigene: 437060 Human

UniProtKB/Swiss-Prot: P99999.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

细胞色素C（cytC）是一种电子传递链蛋白为线粒体呼吸链必须的成份之一。在哺乳动物细胞中，如此高度保守性蛋白常分布在线立体内膜。
新近研究证明细胞浆中细胞色素C为激活细胞调亡所必需的因子。在调亡的过程中，细胞色素C从线立体膜被易位到细胞浆，由细胞色素C激活Caspase-3（CPP32）。
细胞色素C的易位可被过量表达的Bcl-2阻断。细胞色素B与细胞色素C1和Rieske蛋白相结合而形成复合物III（也称细胞色素B-C1复合物）参与细胞呼吸链。该蛋白动物种属间同源性较高；如：猪、犬、牛、鸡、豚鼠等。

产品图片: Sample:
Heart(Rat)A549 Cell Lysate at 40 ug
Primary: Anti-Cytochrome C (bs-0013R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12.8/26 kD
Observed band size: 26 kD
Sample: Cerebellum (Mouse) lysate at 30ug;
Primary: Anti-Cytochrome C (bs-0013R) at 1:300;
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size:12.8/26 kD
Observed band size:27 kD
Sample: Brain (Mouse) lysate at 30ug;
Primary: Anti-Cytochrome C (bs-0013R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size:12.8/26 kD
Observed band size:28 kD
Sample: Hela Cell lysate at 30ug;
Primary: Anti-Cytochrome C (bs-0013R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size:12.8/26 kD
Observed band size:26 kD
Sample:
heart (Mouse) Lysate at 40 ug
Primary: Anti-Cytochrome C (bs-0013R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13/28 kD
Observed band size: 13/28 kD
Blank control: HepG2(blue).
Primary Antibody:Rabbit Anti-Cytochrome C antibody (bs-0013R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody (bs-0013R, 1μg /1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Acquisition of 20,000 events was performed.