Skp2 (D3G5) XP® Rabbit mAb【科瑞奥博】

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Skp2 (D3G5) XP® Rabbit mAb

货号： 2652T 基本售价： 1550.0 元规格： 20ul

产品信息

概述
货号	2652T
同种亚型	Rabbit IgG
反应种属	Human/Monkey
来源宿主	Rabbit
应用	W/IP/IHC-P/IF-IC
目标/特异性	Skp2 (D3G5) XP® Rabbit mAb recognizes endogenous levels of total Skp2 protein. This antibody is predicted to cross-react with Skp2α and Skp2β.
使用方法	WB(1:1000) IP (1:50) IHC-P (1:200) IF-IC (1:800)

性能
供应商	CST
灵敏度	Endogenous
背景	Members of the F-box family of proteins are characterized by the approximate 40 amino acid F-box motif named after cyclin F (1,2). F-box proteins constitute one of the four subunits of the Skp1-Cullin-F-box (SCF) ubiquitin ligase complex. The substrate specificity of SCF complexes is determined by the interchangeable F-box proteins, which act as adaptors by associating with phosphorylated substrate proteins and recruiting them to the SCF core. F-box proteins contain two fundamental domains: the F-box motif mediates binding to Skp1 and a leucine rich repeat (LRR) domain mediates substrate interactions.
存放说明	-20C
计算分子量	48
参考文献	1 . Pagano, M. (2004) Mol Cell 14, 414-6. 2 . Reed, S.I. (2003) Nat Rev Mol Cell Biol 4, 855-64. 3 . Zhang, H. et al. (1995) Cell 82, 915-25. 4 . Nakayama, K. et al. (2004) Dev Cell 6, 661-72. 5 . Bornstein, G. et al. (2003) J Biol Chem 278, 25752-7. 6 . Tedesco, D. et al. (2002) Genes Dev 16, 2946-57. 7 . Bloom, J. and Pagano, M. (2003) Semin Cancer Biol 13, 41-7. 8 . Rodier, G. et al. (2008) EMBO J 27, 679-91. 9 . Gao, D. et al. (2009) Nat Cell Biol 11, 397-408. 10 . Cen, B. et al. (2010) J Biol Chem 285, 29128-37.

参考图片

Confocal immunofluorescent analyis of HeLa cells expressing either a non-targeting shRNA (shNT; left) or shSkp2 (right) using Skp2 (D3G5) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red) (Cell lines kindly provided by Dr. Wenyi Wei, Harvard Medical School.)Confocal immunofluorescent检测表达non-targeting shRNA (shNT; left) 或 shSkp2 (right) 的HeLa细胞，采用Skp2 (D3G5) XP® Rabbit mAb (绿色)。内参采用DY-554 phalloidin (红色)标记（细胞系由来自Harvard Medical School的Dr. Wenyi Wei友情提供）。

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Skp2 (D3G5) XP® Rabbit mAb.Immunohistochemical检测石蜡包埋的人卵巢癌组织，采用Skp2 (D3G5) XP® Rabbit mAb。

Western blot analysis of extracts from various cell lines using Skp2 (D3G5) XP® Rabbit mAb. Western blot检测多种细胞提取物。

Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a Myc/DDK-tagged cDNA expression construct encoding full-length human Skp2 (hSkp2-Myc/DDK, +), using Skp2 (D3G5) XP® Rabbit mAb. Western blot检测mock transfected (-)或转染表达编码人全长Skp2的Myc/DDK-tagged cDNA (hSkp2-Myc/DDK, +)的293T细胞，采用Skp2 (D3G5) XP® Rabbit mAb。

Western blot analysis of extracts from HeLa cells expressing either non-targeting shRNA (shNT) or shSkp2, using Skp2 (D3G5) XP® Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).Western blot检测表达non-targeting shRNA (shNT)或shSkp2的Hela细胞，采用Skp2 (D3G5) XP® Rabbit mAb (upper)和GAPDH (D16H11) XP® Rabbit mAb #5174 (lower)。

Immunohistochemical analysis of paraffin-embedded human lymphoma using Skp2 (D3G5) XP® Rabbit mAb.Immunohistochemical检测石蜡包埋的人淋巴瘤，采用Skp2 (D3G5) XP® Rabbit mAb。

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Skp2 (D3G5) XP® Rabbit mAb.Immunohistochemical检测石蜡包埋的人肺癌组织，采用Skp2 (D3G5) XP® Rabbit mAb。