| 货号 | 14975T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-F |
| 目标/特异性 | β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb recognizes recombinant pE3 form of β-amyloid peptides. This antibody does not cross-react with the non-pyroglutamate (E3) form of β-amyloid peptides. |
| 使用方法 | WB(1:1000) IHC-P (1:100) IF-F (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimers disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). Phosphorylation at Thr668 (a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell-cycle dependent and peaks during G2/M phase (4). APP phosphorylated at Thr668 exists in adult rat brain and correlates with cultured neuronal differentiation (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 4 |
| 参考文献 | 1 . Selkoe, D.J. (1996) J Biol Chem 271, 18295-8. 2 . Caporaso, G.L. et al. (1992) Proc Natl Acad Sci USA 89, 3055-9. 3 . Hung, A.Y. and Selkoe, D.J. (1994) EMBO J 13, 534-42. 4 . Suzuki, T. et al. (1994) EMBO J 13, 1114-22. 5 . Ando, K. et al. (1999) J Neurosci 19, 4421-7. 6 . Iijima, K. et al. (2000) J Neurochem 75, 1085-91. 7 . Jawhar, S. et al. (2011) J Biol Chem 286, 38825-32. 8 . Saido, T.C. et al. (1995) Neuron 14, 457-66. 9 . Saido, T.C. et al. (1996) Neurosci Lett 215, 173-6. 10 . He, W. and Barrow, C.J. (1999) Biochemistry 38, 10871-7. 11 . Demattos, R.B. et al. (2012) Neuron 76, 908-20. |
Confocal immunofluorescent analysis of wild-type (left) and Tg2576 mouse model of Alzheimers brain (right) using β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb (green) and β3-Tubulin (TU-20) Mouse mAb #4466 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded Tg2576 mouse model of Alzheimers brain using β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human Alzheimers brain using β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb. | |
Western blot analysis of the indicated amounts of human Aβ (pE3-42) and Aβ (1-42) peptides using β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb (upper) and β-Amyloid (1-42 Specific) (D3E10) Rabbit mAb #12843 (lower). | |
β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb specificity is demonstrated using peptide dot blot. β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb binds to pre-coated β-amyloid (pE3) peptide but not to β-amyloid (E3) peptide or scrambled β-amyloid (pE3) peptide. |
