Apaf-1 Pure 24/Apaf-1 50ug
货号：
611364 基本售价：
3726.0 元 规格：
-
产品信息
概述| 货号 | 611364 |
| 描述 | The process of apoptosis requires the activation of aspartate-specific cystenine proteases in the caspase family. Group I caspases (1,4,5) cleave at (W/L)EHD tetrapeptide motifs, while group II caspases (2,3,7) cleave the DEXD tetrapeptide motif. Group III caspases (6,8,9) are activators of other caspases via cleavage of (I/V)EXD tetrapeptide sequences. Apoptotic protease-activating factor-1(Apaf-1), cytochrome c, and dATP activate caspase-9, which in turn, initiates the post-mitochondrial-mediated caspase cascade that includes caspase-2, 3, 6, 7, 8 and 10. Apaf-1 is a soluble protein with a short N-terminal caspase recruitment domain (CARD), a central CED-4 homology domain, and 12 WD-40 repeats that may be involved in protein-protein interactions. During apoptosis, a large (700 kDa) aposome complex containing Apaf-1, cytochrome c, caspase-3, 7, and 9, and a smaller (200-300 kDa) microaposome complex containing caspase-3 and 7 exhibit higher cleavage activity than "free" caspase heterotetramers. Thus Apaf-1 is a component of the large aposome complex, which functions in caspase activation leading to caspase-dependent proteolytic events and apoptosis. |
| 别名 | Apoptotic Protease Activating Factor-1 |
| 同种亚型 | IgG1 |
| 反应种属 | Human (QC Testing) |
| 来源宿主 | Mouse |
| 使用方法 | WB, IF |
性能| 供应商 | BD Pharmingen |
| 免疫原 | Human Apaf-1 aa. 252-445 |
| 存放说明 | The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at -20°C.
|
| 存储溶液 | Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide. |
| 浓度 | 250 µg/ml |
