PKA RIIBta (pS114) Pure 47/PKA 50ug
货号：
612550 基本售价：
5184.0 元 规格：
50ug
产品信息
概述| 货号 | 612550 |
| 描述 | cAMP-dependentProteinK inase (PKA) is composed of two distinct subunits: catalytic (C) and regulatory ( R). Four regulatory subunits have been identified: RIα, RIβ, RIIα, and RIIβ. These subunits define type I and II PKAs. Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active. Type I and type II holoenzymes have three potential C subunits (Cα, Cβ, or Cγ). Most cells, including T lymphocytes, express both type I and type II PKAs. RII a expression is associated with cellular transformation, while RIIβ expression correlates with mitotic arrest and cellular differentiation. Type II PKA can be distinguished by autophosphorylation of the R subunits, while type I PKA binds Mg/ATP with high affinity. The cAMP-dependent autophosphorylation of t hehuman RIIβ subunits occurs at serine 114 (S114). In addition to their enzyme regulatory activity, the RII α and RII β subunits determine the subcellular location of the holoenzymes via their interactions with specific intracellular anchoring proteins. The 47/PKA monoclonal antibody recognizes the phosphorylated S114 in the RIIβ subunit of PKA. The orthologous phosphorylation site in mouse and rat PKA[RIIβ] is S112. |
| 同种亚型 | IgG1 |
| 反应种属 | Rat (QC Testing) Human, Mouse (Tested in Development) |
| 来源宿主 | Mouse |
| 使用方法 | WB, IC/FC |
性能| 供应商 | BD Pharmingen |
| 免疫原 | Phosphorylated Human PKA[RIIβ] peptide Peptide |
| 存放说明 | The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at -20°C.
|
| 存储溶液 | Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide. |
| 浓度 | 250 µg/ml |
