PKA[RII Bta] (pS114) Alexa 488 47 50Tst
货号：
560204 基本售价：
3920.0 元 规格：
50Tst
产品信息
概述| 货号 | 560204 |
| 描述 | cAMP-dependentProteinK inase (PKA) is composed of two distinct subunits: catalytic (C) and regulatory ( R). Four regulatory subunits have been identified: RIα, RIβ, RIIα, and RIIβ. These subunits define type I and II PKAs. Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active. Type I and type II holoenzymes have three potential C subunits (Cα, Cβ, or Cγ). Most cells, including T lymphocytes, express both type I and type II PKAs. RII a expression is associated with cellular transformation, while RIIβ expression correlates with mitotic arrest and cellular differentiation. Type II PKA can be distinguished by autophosphorylation of the R subunits, while type I PKA binds Mg/ATP with high affinity. The cAMP-dependent autophosphorylation of t hehuman RIIβ subunits occurs at serine 114 (S114). In addition to their enzyme regulatory activity, the RII α and RII β subunits determine the subcellular location of the holoenzymes via their interactions with specific intracellular anchoring proteins. The 47/PKA monoclonal antibody recognizes the phosphorylated S114 in the RIIβ subunit of PKA. The orthologous phosphorylation site in mouse and rat PKA[RIIβ] is S112. |
| 别名 | KAP3, PKAR2B, PRKAR2B |
| 同种亚型 | IgG1 |
| 反应种属 | Human (QC Testing) Rat (Tested in Development) Mouse (Predicted) |
| 来源宿主 | Mouse |
| 使用方法 | IC/FC |
性能| 供应商 | BD Pharmingen |
| 免疫原 | Phosphorylated Human PKA[RIIβ] peptide Peptide |
| 标记 | Alexa Fluor 488 |
| 存放说明 | Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
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| 存储溶液 | Aqueous buffered solution containing BSA and ≤0.09% sodium azide. |
