ERK2 Alexa 647 G263-7 50Tst
货号：
558526 基本售价：
3920.0 元 规格：
50Tst
产品信息
概述| 货号 | 558526 |
| 描述 | The members of the Mitogen-ActivatedProteinK inase (MAPK) family are components of a key signal transduction cascade that links events at the cell surface to responses in the nucleus. The signaling cascade is found in species as varied as yeast and humans, with many of the proteins being well conserved. In mammals the most widely studied members of the cascade are the E xtracellular signal- RegulatedK inases, ERK1 (p44 MAPK) and ERK2 (p42 MAPK). ERK1 and ERK2 share 85% homology and are activated by extracellular signals such as growth factors, hormones, and phorbol esters. Activation occurs through a series of phosphorylations by kinases activating other kinases and eventually leading to phosphorylation of the ERKs. Growth factor stimulation leads to activation of Ras and Raf, leading to phosphorylation of MEK1 (MAPK/ERK kinase) which, in turn, activates the ERKs via dual phosphorylation. Once activated, the ERKs phosphorylate other cytoplasmic signaling molecules (protein kinases and phosphatases), cell-surface receptors, microtubule-associated proteins, and transcription factors in the nucleus. Thus, the active ERK has myriad downstream effectors that implicate it in the control of cell proliferation and differentiation, as well as regulation of the cytoskeleton. Furthermore, studies have shown that elevated ERK activity is associated with some cancers. The G263-7 recognizes ERK2. It does not cross-react with ERK1. Clone G263-7 was originally characterized in human (A431) and mouse (NIH/3T3) cells. A human ERK2 synthetic peptide was used as immunogen. |
| 同种亚型 | IgG1,κ |
| 反应种属 | Human, Mouse (QC Testing) |
| 来源宿主 | Mouse |
| 使用方法 | IC/FC |
性能| 供应商 | BD Pharmingen |
| 免疫原 | Human ERK2 Synthetic Peptide |
| 标记 | Alexa Fluor 647 |
| 存放说明 | Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
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| 存储溶液 | Aqueous buffered solution containing BSA and ≤0.09% sodium azide. |
