JNK (PT183/PY185) Alexa 647 N9-66 50Tst
货号：
562481 基本售价：
3675.0 元 规格：
50Tst
产品信息
概述| 货号 | 562481 |
| 描述 | The N9-66 monoclonal antibody specifically binds to JNK1 and JNK2 phosphorylated at the pT183/pY185 sites. c-Jun NH2-terminal Kinases (JNKs), also called Stress Activated Protein Kinases (SAPKs), are mitogen-activated protein kinases (MAPKs) with observed molecular weights of ~46 kDa (JNK1) and ~54 kDa (JNK2). Along with the p38 and ERK families, JNK represents one of three major classes of MAPKs. Complete activation of JNK requires the phosphorylation of both Thr183 and Tyr185 that are located in a Thr-X-Tyr motif. Phosphorylation of these residues is carried out by MKK4 and MKK7 that are phosphorylated and activated by MEKKs and MLKs in response to stress signals delivered through small GTPases of the Rho family. Once activated, JNK can translocate into the nucleus and regulate the expression of genes through phosphorylation of c-Jun, ATF-2, and other transcription factors. JNK plays a role in signal transduction in response to cytokines and various forms of environmental stress, such as endotoxins, UV irradiation, heat, and hyperosmolarity. JNK is critical to the regulation of cell growth, apoptosis, and the cellular response to stress, making it an important factor in tumorigenesis and adaptive immunity. During antibody development, the N9-66 monoclonal antibody was found to detect phosphorylated JNK1/2 by Western blot analysis of cellular lysates and by immunofluorescent staining and flow cytometric analysis of fixed and permeabilized cells. This antibody crossreacts with phosphorylated JNK1/2 expressed by mouse cells, as tested by Western blot analysis and flow cytometry. |
| 别名 | MAPK8, MAPK9; SAPK1, SAPK; PRKM8, PRKM9 |
| 同种亚型 | Mouse IgG1, κ |
| 反应种属 | Human (QC Testing) Mouse (Tested in Development) |
性能| 供应商 | BD Pharmingen |
| 免疫原 | Phosphorylated Human JNK Peptide |
| 标记 | Alexa Fluor 647 |
| 存放说明 | The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
|
| 存储溶液 | Aqueous buffered solution containing BSA and ≤0.09% sodium azide. |
