elF4E (pS209) PE J77-925 50Tst
货号：
560229 基本售价：
3675.0 元 规格：
50Tst
产品信息
概述| 货号 | 560229 |
| 描述 | Theeukaryotic translation InitiationFactor4E (eIF4E) is a 25-kDa phosphoprotein that specifically binds to the 7-methylguanosine-containing cap of mRNA. eIF4E is the rate-limiting component for the initiation of cap-dependent translation by the eIF4F translation initiation complex, which is composed of eIF4E, eIF4G, and eIF4A. This complex promotes the unwinding of secondary structure at the 5 untranslated region of mRNA that is necessary to expose and locate the AUG initiation codon. Other functions of eIF4E have been identified, such as promoting the export of mRNAs that are involved in cell cycle progression from the nucleus and differentially regulating the translation of certain mRNAs in the cytoplasm. Three mechanisms for eIF4E regulation have been identified:Mnk1-mediated phosphorylation on serine 209 (S209) is required for eIF4E binding to the cap structure; over-expression of phosphorylated eIF4E can lead to increased cell proliferation, suppression of apoptosis, and a transformed phenotype; and interactions with nonphosphorylated eIF4E-binding proteins inhibit the formation of the eIF4F complex. The J77-925 monoclonal antibody recognizes the phosphorylated S209 (pS209) of eIF4E. |
| 别名 | eIF-4E, CBP, EIF4E1, EIF4EL1, EIF4F, mRNA cap-binding protein |
| 同种亚型 | IgG1,κ |
| 反应种属 | Human (QC Testing) Mouse, Rat (Predicted) |
| 来源宿主 | Mouse |
| 使用方法 | IC/FC |
性能| 供应商 | BD Pharmingen |
| 免疫原 | Phosphorylated Human eIF4E Peptide |
| 标记 | PE |
| 存放说明 | Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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| 存储溶液 | Aqueous buffered solution containing BSA and ≤0.09% sodium azide. |
