CD22 (pY822) Alexa 488 12a/CD22 50Tst
货号：
560075 基本售价：
3675.0 元 规格：
50Tst
产品信息
概述| 货号 | 560075 |
| 描述 | CD22 is a member of the immunoglobulin (Ig) superfamily that contains several extracellular Ig domains and cytoplasmic ImmunoreceptorTyrosine-basedInhibitoryM otifs (ITIMs). It is expressed in the cytoplasm of pro-B and pre-B cells and on the surface of IgD-positive mature B lymphocytes. Differential splicing of the human CD22 gene results in the expression of two isoforms; the full-length CD22 b has 7 Ig domains, while CD22 a has only 5. CD22 b is the predominant isoform in human cells, at both the mRNA and protein levels. Ligands for the extracellular domains of CD22 are sialic acid-linked glycoproteins on the B cell surface. The intracellular ITIM motifs are substrates of src family tyrosine kinases. Signal transduction is initiated by the phosphorylation of tyrosines in the ITIMs of CD22 upon ligation of the B cell receptor (BCR). Tyrosine phosphorylation in ITIM3 of human CD22 or ITIM2 of mouse CD22 is required for recruitment of the protein tyrosine phosphatase PTP1C (SHP-1), which may terminate signal transduction by CD22 and by co-localized receptors, such as the BCR. Studies of mice deficient for CD22 suggest that this adhesion molecule generates both negative and positive regulatory signals that affect B cell development and responsiveness. The 12a/CD22 monoclonal antibody recognizes the phosphorylated tyrosine 822 (pY822) in ITIM3 of human CD22 b . The equivalent site in human CD22 a is pY645. The orthologous phosphorylation site in the predominant isoform of mouse CD22 is pY837, which is in ITIM2. |
| 别名 | Human BL-CAM, Siglec-2, or Leu-14 (pY822); Mouse Lyb-8 (pY837) |
| 同种亚型 | IgG1,κ |
| 反应种属 | Mouse (QC Testing) |
| 来源宿主 | Mouse |
| 使用方法 | IC/FC |
性能| 供应商 | BD Pharmingen |
| 免疫原 | Phosphorylated Mouse CD22 Peptide |
| 标记 | Alexa Fluor 488 |
| 存放说明 | Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
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| 存储溶液 | Aqueous buffered solution containing BSA and ≤0.09% sodium azide. |
