NF-KpaB p65 (pS529) PE-Cy7 50Tst
货号：
560335 基本售价：
3555.0 元 规格：
50Tst
产品信息
概述| 货号 | 560335 |
| 描述 | Nuclear factor k B (NF- k B) is a ubiquitously expressed transcription factor that regulates the expression of 200-300 genes. It is crucial for basic cellular responses to stress and pathogens, such as proliferation, survival, development, and apoptosis. The most studied NF- k B complex consists of the p50 (also known as NF- k B1) and p65 (also known as REL-A) subunits, both containing a 300-amino acid region with homology to the Rel proto-oncogene product (RH domain). The RH domain contains motifs for dimerization, nuclear localization, and binding to specific DNA sequences. In addition to the RH domain, the p65 subunit contains the transactivation domain, which is responsible for the interaction with the inhibitor I k B and which contains phosphorylation sites. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to I k B. This complex prevents nuclear translocation and activity of NF- k B. In response to stimuli such as cytokines, LPS, DNA damage, and viral infections, I k B is phosphorylated at critical residues. This phosphorylation induces dissociation of the I kB/NF-k B complex, allowing the free heterodimeric NF- k B to translocate to the nucleus. Furthermore, optimal activation of NF- k B requires phosphorylation in the transactivation domain of p65. In the nucleus, activated NF- k B dimers bind to the k B sites within promoters and enhancers and function as transcriptional activators. The K10-895.12.50 monoclonal antibody recognizes the phosphorylated serine 529 (pS529) in the transactivation domain of human NF- k B p65 subunit. |
| 别名 | transcription factor p65, RELA, MGC131774, NFKB3 |
| 同种亚型 | IgG2b,κ |
| 反应种属 | Human (QC Testing) |
| 来源宿主 | Mouse |
| 使用方法 | IC/FC |
性能| 供应商 | BD Pharmingen |
| 免疫原 | Phosphorylated Human NF-κB p65 Peptide |
| 标记 | PE-Cy7 |
| 存放说明 | Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.The antibody was conjugated with PE-Cy7 under optimum conditions, and unconjugated antibody and free PE-Cy7 were removed.
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| 存储溶液 | Aqueous buffered solution containing BSA and ≤0.09% sodium azide. |
