PLC Gma1 (pY783) Alexa 488 27/PLC 50Tst
货号：
557884 基本售价：
3555.0 元 规格：
50Tst
产品信息
概述| 货号 | 557884 |
| 描述 | ThePhospholipaseC (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol. The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C. Within the PLC family, PLC g is the only member that contains SH2 and SH3 domains. These domains enable it to interact with receptor tyrosine kinases and become enzymatically activated via phosphorylation. It exists as two isoforms: 1) PLC g 1, which is ubiquitously expressed, and 2) PLC g 2, found primarily in the lymphoid system. PLC g is essential for growth factor-induced cell motility and mitogenesis. PLC g 1-null mice exhibit retarded embryonic growth and lethality in midgestation. In addition, PDGF stimulation leads to phosphorylation of PLC g1 at Tyr 783 and activation of hydrolyzing activity. Overexpression of PLC g is evident in several forms of cancer, and it has been identified as a key mediator of PDGF-dependent cellular transformation. Thus, regulation of PLC g activity by growth factors is involved in cell growth and transformation. The 27/PLC antibody recognizes PLC g 1 phosphorylated at Y783. The fluorochrome-conjugated formats have been evaluated using a human model system. However, the unconjugated form of this antibody (Cat. no. 612464) has been shown to react with human, mouse, and rat cell lysates by western blot. |
| 同种亚型 | IgG1 |
| 反应种属 | Human (QC Testing) |
| 来源宿主 | Mouse |
| 使用方法 | IC/FC |
性能| 供应商 | BD Pharmingen |
| 免疫原 | Phosphorylated peptide including the tyrosine 783 of human PLCγ1 |
| 标记 | Alexa Fluor 488 |
| 存放说明 | Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
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| 存储溶液 | Aqueous buffered solution containing BSA and ≤0.09% sodium azide. |
