| 货号 | 2697L |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IHC-F/F |
| 目标/特异性 | Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb detects IKKα only when phosphorylated at Ser176/180 and IKKβ only when phosphorylated at Ser177/181. |
| 使用方法 | WB(1:1000) IHC-P (1:150) F (1:3200) IHC-F (1:150) |
| 供应商 | CST |
| 背景 | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation; Ser177 and Ser181 in the activation loop of IKKβ (serine 176 and 180 in IKKα) are the specific sites whose phosphorylation causes conformational changes resulting in kinase activation (10-13). |
| 存放说明 | -20C |
| 计算分子量 | 85 IKK-alpha 87 IKK-beta |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic localization, using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb.免疫组织化学染色分析石蜡包埋人结肠癌组织，图中所示为细胞质的定位。所用抗体为Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb in the presence of control peptide (left) or Phospho-IKK-alpha/beta (Ser176/180) Blocking Peptide #1023 (right).免疫组织化学染色分析石蜡包埋人乳腺癌组织。所用抗体为Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb, 在对照多肽 (左图) 或Phospho-IKK-alpha/beta (Ser176/180) 封闭多肽#1023 (有图)存在下。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, untreated (left) or λ phosphatase-treated (right), using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb.免疫组织化学染色分析石蜡包埋未经处理 (左图) 和经λ phosphatase处理 (右图) 人结肠癌组织。所用抗体为Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human gall bladder (chronic cholecystitis), using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb.免疫组织化学染色分析石蜡包埋人胆囊（慢性胆囊炎）组织。所用抗体为Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human lung (chronic bronchitis), using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb.免疫组织化学染色分析石蜡包埋人慢性支气管炎组织。所用抗体为Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb。 | |
Western blot analysis of extracts from TNF-alpha and Calyculin A treated HeLa and NIH/3T3 cells, using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb.Western免疫印迹。用Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb 研究经TNF-alpha 和 Calyculin A处理的HeLa 和 NIH/3T3 细胞的细胞提取液。 | |
Immunohistochemical analysis of frozen H1650 xenograft, showing cytoplasmic localization using Phospho-IKKα/β (Ser176/180)(16A6) Rabbit mAb.免疫组织化学染色研究冰冻H1650 异种移植物，图片显示的是细胞质的定位，所用的抗体为Phospho-IKKα/β (Ser176/180)(16A6) Rabbit mAb。 | |
Western blot analysis of extracts from THP-1 cells, differentiated with TPA (#9905, 80 nM for 24h) and treated with 1 μg/ml LPS for the indicated times, using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb.Western免疫印迹。用Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb研究用TPA #9905, 80 nM 分化24小时后经1 μg/ml LPS 处理一定时间的 THP-1细胞的细胞提取液。 | |
Flow cytometric analysis of THP-1 cells, untreated (blue) and with TPA and LPS (green) using IKK-α (Ser176/Ser180) phosphate Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (PE Conjugate) #8885 was used as a secondary antibody. |
