| 货号 | 9148S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625. |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | IF-IC/F |
| 使用方法 | F(1:50) IF-IC (1:50) |
| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). |
| 存放说明 | 4C |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with staurosporine #9953 (1 μM for 3 hours, right), using Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red). 共聚焦免疫荧光分析HeLa细胞，未处理（左）和Staurosporine #9953（1 μM, 3 hr；右）处理，使用抗体为 Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb兔单抗 (Alexa Fluor ® 488 Conjugate) （绿色）。DY-554 phalloidin（红色）标记肌动蛋白丝。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 | |
Flow cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate). |
