| 货号 | 13708S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | IF-IC/F |
| 使用方法 | F(1:50) IF-IC (1:50) |
| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 (6). HS1 also plays an important role in platelet activation (7). |
| 存放说明 | 4C |
Flow cytometric analysis of Ramos cells, untreated (blue) or IgM-treated (green), using Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate). | |
Immunofluorescent analysis of Ramos cells untreated (left) or treated with IgM (12 μg/mL, 10 min, right) using Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) (red). Blue pseudocolor= ProLong® Gold Antifade Reagent with DAPI #8961. |
