| 货号 | 3300S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:50) F (1:1600) IF-IC (1:2000) |
| 供应商 | CST |
| 背景 | Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to effect progression through the restriction point and pRb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of its protein expression and through phosphorylation-dependent degradation (4).细胞周期蛋白依赖性蛋白激酶CDK4和CDK6的活性受三个因素的调控：T-环路磷酸化，周期蛋白伴侣(D-型周期蛋白)的丰度，与CDK抑制剂Cip/Kip 或 INK蛋白家族的关系(1)。失活的三元复合物cyclin D/CDK4 和p27 Kip1需要胞外有丝分裂刺激因子参与p27伴侣的释放和降解，伴随周期蛋白D的水平升高，通过限制点和pRb依赖性的S期进入影响细胞周期的进程(2)。活跃的cyclin D/CDK4复合体靶向视网膜母细胞瘤蛋白以利于磷酸化，促进可以激活G1/S期基因表达的转录因子E2F的释放(3)。生长因子退出后，通过下调蛋白表达和磷酸化依赖性蛋白降解，周期蛋白D的水平下降(4)。 |
| 存放说明 | -20C |
| 计算分子量 | 36 |
Western blot analysis of extracts from HT-1080 cells, untreated or treated with MG132 (10 μM, 4 hours), using Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb. western blot方法分析未处理或MG132处理的 (10 μM, 4 hours)HT-1080细胞提取物，使用的抗体为Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb。 | |
Western blot analysis of extracts from HT-1080 cells, treated with MG132 and with or without λ phosphatase, using Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb (upper) or Cyclin D1 (DC56) Mouse mAb #2926 (lower). western blot方法检测加入或未加入λ磷酸酶的情况下，经MG132处理的HT-1080细胞提取物，使用的抗体为Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb（上图）或Cyclin D1 (DC56) Mouse mAb #2926 (下图)。 | |
Flow cytometric analysis of HT-1080 cells, untreated (blue) or MG132-treated (green), using Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb.流式细胞术分析未处理（蓝色）或MG-132处理的（绿色）HT-1080细胞，使用的抗体为Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb。 | |
Confocal immmunofluorescent analysis of HT-1080 (top) and Saos-2 cells (bottom), untreated (left) or treated with MG132 alone (center) or with MG132 followed by λ-phosphatase (right), using Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). 激光共聚焦荧光法检测HT-1080 (上) and Saos-2 cells (下), 左图为对照细胞，中间图为MG132单独处理，右图为MG132处理后加入λ磷酸酶，检测抗体为Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb，呈绿色。肌动蛋白丝以DY-554 phalloidin标记，为红色。 |
