| 货号 | 8714S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb recognizes endogenous levels of HS1 protein only when phosphorylated at Tyr397. |
| 使用方法 | WB(1:1000) F (1:200) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 (6). HS1 also plays an important role in platelet activation (7). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
Western blot analysis of extracts from Ramos cells, serum starved for 12 hours, then treated with anti-IgM antibody or λ phosphatase, using Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb (upper), or HS1 Antibody (Human Specific) #4503 (lower).Western免疫印迹。用Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb (上图) 或 HS1 Antibody (人特异性) #4503 (下图) 研究血清饥饿12小时并经anti-IgM antibody 或 λ phosphatase 处理的Ramos 细胞的细胞提取液。 | |
Confocal immunofluorescent analysis of Ramos cells, untreated (left) or treated with IgM (12 μg/mL, 10 min, right) using Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析(左图) 或 经IgM (12 μg/mL, 10 min, 右图)处理的Ramos细胞，所用抗体为Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb (绿色)。Blue pseudocolor= DRAQ5® #4084 (DNA荧光染料) | |
Flow cytometric analysis of Ramos cells, untreated (blue) or IgM-treated (green), using Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb.流式细胞仪分析未经处理(蓝色) 或经IgM处理(绿色)的Ramos细胞,所用抗体为Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb。 |
