| 货号 | 3520S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/F |
| 使用方法 | WB(1:1000) F (1:100) |
| 供应商 | CST |
| 背景 | Nucleophosmin (NPM; also known as B23, numatrin or NO38) is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication and molecular chaperoning activities (1,2). The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase (3,4).核磷蛋白(NPM；又称B23、Numatrin或N038)是一种高丰度的核仁磷酸化蛋白。它拥有几个独特的细胞职能，包括核糖体组装和运输，胞质/胞核运输，DNA聚合酶α活性的调节，中心体复制和分子伴侣活性(1,2)。NPM的编码基因也因为和间变性淋巴瘤激酶(ALK)酪氨酸受体激酶的融合而广为人知。NPM部分通过提供一个二聚体结构域促进转化，最终激活融合激酶(3,4)。 |
| 存放说明 | -20C |
| 计算分子量 | 38 |
Western blot analysis of extracts of HeLa cells, either untreated or nocodazole-treated, using Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb (upper). Lower panel shows total NPM using NPM Antibody #3542.western blot方法检测未处理和nocodazole处理的HeLa细胞提取物，使用的抗体为Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb (上图)。下图示NPM总蛋白，使用的抗体为NPM Antibody #3542。 | |
Flow cytometric analysis of Jurkat cells using Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb compared to Propidium Iodide (PI)/RNase Staining Solution #4087. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. |
