| 货号 | 5483S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | Phospho-TBK1 (Ser172) (D52C2) XP® Rabbit mAb detects endogenous levels of TBK1 only when phosphorylated at Ser172. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | TBK1 (TANK-binding kinase 1)/NAK (NF-κB activating kinase) is an IκB kinase (IKK)-activating kinase and can activate IKK through direct phosphorylation (1). TBK1 was identified through association with the TRAF binding protein, TANK, and found to function upstream of NIK and IKK in the activation of NF-κB (2). TBK1/NAK induces IκB degradation and NF-κB activity through IKKβ. NAK may mediate IKK and NF-κB activation in response to growth factors that stimulate PKCε activity (1). TBK1 plays a pivotal role in the activation of IRF3 in the innate immune response (3). |
| 存放说明 | -20C |
| 计算分子量 | 84 |
Confocal immunofluorescent analysis of THP-1 cells differentiated with TPA #4174 (80nM, overnight) (left), followed by treatment with LPS (1μg/ml, 1 hour) (center) or LPS with λ phosphatase treatment (right) using Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 Phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析经不同处理的THP-1细胞，TPA #4174 (80nM,过夜) (左图), 经TPA处理后又经LPS (1μg/ml, 1小时)(中间图) 或 LPS 与λ磷酸酶处理(右图)，所用抗体为Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb (绿色)。肌动蛋白丝是用DY-554 Phalloidin 标记(红色)。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)。 | |
Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, overnight) followed by treatment with LPS (1 μg/ml, 1 hour), with or without phosphatase treatment using Phospho-TBK1 (Ser172) (D52C2) XP® Rabbit mAb (upper), or total TBK1/NAK (D1B4) Rabbit mAb #3504 (lower).Western免疫印迹分析THP-1细胞的细胞抽提液，THP-1细胞经过 TPA #4174 (80 nM, 过夜)分化然后用在磷酸酶存在或不存在下用LPS (1 μg/ml, 1小时)处理 所用抗体为Phospho-TBK1 (Ser172) (D52C2) XP® Rabbit mAb (上图) 或 total TBK1/NAK (D1B4) Rabbit mAb #3504 (下图)。 | |
Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, overnight) followed by treatment with LPS (1 μg/ml), up to 24h, using Phospho-TBK1 (Ser172) (D52C2) XP® Rabbit mAb (upper), or total TBK1/NAK (D1B4) Rabbit mAb #3504 (lower).Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, overnight) followed by treatment with LPS (1 μg/ml), up to 24h, using Phospho-TBK1 (Ser172) (D52C2) XP® Rabbit mAb (upper), or total TBK1/NAK (D1B4) Rabbit mAb #3504 (lower).Western免疫印迹分析THP-1细胞的细胞抽提液，THP-1细胞经过 TPA #4174 (80 nM, 过夜)分化然后用LPS (1 μg/ml)处理多达24小时，所用抗体为Phospho-TBK1 (Ser172) (D52C2) XP® Rabbit mAb (上图)或total TBK1/NAK (D1B4) Rabbit mAb #3504 (下图)。 | |
Flow cytometric analysis of THP-1 cells differentiated with TPA #9905, untreated (blue) or LPS-treated (green), using Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb.流式细胞仪分析经过TPA #9905处理的THP-1细胞，未经其他处理的 (蓝色) 或 LPS处理的(绿色), 所用抗体为Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb。 |
