CD71 (D7G9X) XP® Rabbit mAb (PE Conjugate)
货号：
82582S 基本售价：
4264.0 元 规格：
100ul
产品信息
概述| 货号 | 82582S |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | F |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 背景 | Transferrin receptor 1 (CD71, TFRC) is a type II transmembrane receptor and carrier protein responsible for the uptake of cellular iron through receptor-mediated endocytosis (1). Neutral pH at the cell surface promotes binding of the iron-binding glycoprotein transferrin (Tf) to the CD71 receptor. The receptor-ligand complex enters the cell through receptor-mediated endocytosis and is internalized into an endosome. Relatively lower endosomal pH leads to a change in the local charge environment surrounding the iron-transferrin binding site and results in the release of iron (2). The receptor-ligand complex is recycled to the cell surface where transferrin dissociates from the CD71 receptor (2). Ubiquitously expressed transferrin receptor is continuously recycled and undergoes clathrin-mediated endocytosis regardless of ligand binding state. The interaction between receptor and ligand has been studied in detail. The helical domain of CD71 directly interacts with the transferrin C-lobe and induces a conformation change in Tf to facilitate the transport process (3). Interaction between the receptor CD71 and transferrin is mediated by the membrane protein hemochromatosis (HFE). HFE binds the α-helical domain of CD71, blocking formation of the CD71-transferrin complex and inhibiting iron uptake (4,5). In addition to binding transferrin, CD71 also interacts with H-ferritin at the cell surface and transports this intracellular iron storage protein to cellular endosomes and lysosomes (6). Additional studies indicate that the transferrin receptor is an evolutionarily conserved receptor for a number or arenaviruses and at least one retrovirus (7,8). Aberrant expression of CD71 is seen in a number of cancers, including thyroid carcinomas, lymphomas, and T-lineage leukemias, suggesting a possible therapeutic role for targeted inhibition of the transferrin receptor (9,10). |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of human peripheral blood mononuclear cells, untreated (left) or treated with anti-human CD3 (10 μg/ml, 72 hours, 37ºC) and anti-human CD28 (5 μg/ml, 72 hours, 37ºC) using CD71 (D7G9X) XP® Rabbit mAb (PE Conjugate) and co-stained with a CD3 antibody. |
