| 货号 | 8494S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Met (D1C2) XP® Rabbit mAb #8198. |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | IF-IC/F |
| 目标/特异性 | Met (D1C2) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total Met protein. |
| 使用方法 | F(1:50) IF-IC (1:100) |
| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation. Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Research studies have shown that altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast. Thus, investigators have concluded that Met is an attractive potential cancer therapeutic and diagnostic target (6,7). |
| 存放说明 | 4C |
Flow cytometric analysis of K-562 (blue) and HT-29 (green) cells using Met (D1C2) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate).流式细胞术检测K-562细胞 (蓝色)和HT-29 细胞(绿色)，使用的抗体为et (D1C2) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate). | |
Confocal immunofluorescent analysis of HT-29 (left) and T-47D (right) cells using Met (D1C2) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光法检测HT-29 细胞(左图)和T-47D 细胞(右图)，使用的抗体为Met (D1C2) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate)（绿色）。肌动蛋白丝用DY-554鬼笔环肽进行标记（红色）。蓝色伪彩色为DNA荧光燃料，产品信息为DRAQ5® #4084。 |
