| 货号 | 5448S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Nanog (D73G4) XP® Rabbit mAb #4903. |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | IF-IC/F |
| 使用方法 | F(1:50) IF-IC (1:50) |
| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3). |
| 存放说明 | 4C |
Confocal immunofluorescent analysis of NTERA-2 cells (left) and HeLa cells (right) using Nanog (D73G4) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Actin filaments were labeled with DY-554 phalloidin (red).Nanog (D73G4) XP®兔单抗(Alexa Fluor® 647 标记) (蓝色假色)对NTERA-2细胞（左）和HeLa细胞（右）进行激光共聚焦荧光分析. 用DY-554鬼笔环肽（红色）标记肌动蛋白丝。 | |
Flow cytometric analysis of HeLa cells (blue) and NTERA-2 cells (green) using Nanog (D73G4) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate).用Nanog (D73G4) XP®兔单抗(Alexa Fluor® 647 标记) 对HeLa 细胞(蓝色) 和 NTERA-2 细胞(绿色) 进行流式细胞分析。 |
