PDGF Receptor α (D13C6) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate)
货号：
5876S 基本售价：
4264.0 元 规格：
100ul
产品信息
概述| 货号 | 5876S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated PDGF Receptor α (D13C6) XP® Rabbit mAb #5241. |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | PDGF Receptor α (D13C6) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of PDGF receptor α protein. |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | Platelet derived growth factor (PDGF) family proteins exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC, and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor α (PDGFRα) and PDGF receptor β (PDGFRβ). PDGFRα and PDGFRβ share 75% to 85% sequence homology between their two intracellular kinase domains, while the kinase insert and carboxy-terminal tail regions display a lower level (27% to 28%) of homology (1). PDGFRα homodimers bind all PDGF isoforms except those containing PDGF D. PDGFRβ homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGF receptor α/β binds PDGF B, C, and D homodimers, as well as the PDGF AB heterodimer (2). PDGFRα and PDGFRβ can each form heterodimers with EGFR, which is also activated by PDGF (3). Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding (4). Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules, such as GRB2, Src, GAP, PI3 kinase, PLCγ, and NCK. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration, and differentiation (5). Tyr751 in the kinase-insert region of PDGFRβ is the docking site for PI3 kinase (6). Phosphorylated pentapeptides derived from Tyr751 of PDGFRβ (pTyr751-Val-Pro-Met-Leu) inhibit the association of the carboxy-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFRβ (7). Tyr740 is also required for PDGFRβ-mediated PI3 kinase activation (8). |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of HCC827 (blue) or NCI-H1703 (green) cells using PDGF Receptor α (D13C6) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate).流式细胞术方法检测HCC827细胞(蓝色)或NCI-H1703细胞(绿色)，使用的抗体为PDGF Receptor α (D13C6) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate). |
