| 货号 | 23353S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | F |
| 目标/特异性 | Progesterone Receptor A/B (D8Q2J) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of total progesterone receptor A and B proteins. This antibody does not cross-react with either the glucocorticoid receptor or the mineralocorticoid receptor. |
| 使用方法 | F(1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Human progesterone receptor (PR) is expressed as two forms: the full length PR-B and the short form PR-A. PR-A lacks the first 164 amino acid residues of PR-B (1,2). Both PR-A and PR-B are ligand activated, but differ in their relative ability to activate target gene transcription (3,4). The activity of PR is regulated by phosphorylation; at least seven serine residues are phosphorylated in its amino-terminal domain. Three sites (Ser81, Ser102, and Ser162) are unique to full length PR-B, while other sites (Ser190, Ser294, Ser345, and Ser400) are shared by both isoforms (5). Phosphorylation of PR-B at Ser190 (equivalent to Ser26 of PR-A) is catalyzed by CDK2 (6). Mutation of Ser190 results in decreased activity of PR (7), suggesting that the phosphorylation at Ser190 may be critical to its biological function. |
| 存放说明 | 4C |
| 参考文献 | 1 . Evans, R.M. (1988) Science 240, 889-895. 2 . Kastner, P. et al. (1990) EMBO J. 112, 1603-1614. 3 . Giangrande, P.H. et al. (2000) Mol. Cell. Biol. 20, 3102-3115. 4 . Wen, D.X. et al. (1994) Mol. Cell. Biol. 14, 8356-8364. 5 . Clemm, D.L. et al. (2000) Mol. Endocrinol. 14, 52-65. 6 . Zhang, Y. et al. (1997) Mol. Endocrinol. 11, 823-832. 7 . Takimoto, G.S. et al. (1996) J. Biol. Chem. 271, 13308-13316. |
Flow cytometric analysis of MDA-MB-231 cells (blue) and T47D cells (green) using Progesterone Receptor A/B (D8Q2J) XP® Rabbit mAb (PE Conjugate). |
