| 货号 | 56687S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | F |
| 目标/特异性 | p63-α (D2K8X) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of total p63-α protein. This antibody will detect both the full-length isoform (TAp63-α) and the truncated δNp63-α isoform, but will not recognize the p63-β or p63-γ isoform. |
| 使用方法 | F(1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function (2). While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors (2). Research investigators frequently observe amplification of the p63 gene in squamous cell carcinomas of the lung, head and neck (2,3). The p63 gene contains an alternative transcription initiation site that yields a 40 kDa δNp63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the α, β, and γ isoforms (3,4). |
| 存放说明 | 4C |
| 参考文献 | 1 . Levine, A.J. (1997) Cell 88, 323-31. 2 . Waltermann, A. et al. (2003) Oncogene 22, 5686-93. 3 . Hibi, K. et al. (2000) Proc Natl Acad Sci U S A 97, 5462-7. 4 . Yang, A. et al. (1999) Nature 398, 714-8. |
Flow cytometric analysis of ME-180 (green) and MCF7 (blue) cells using p63-α (D2K8X) XP® Rabbit mAb (PE conjugate). |
