| 货号 | 59999S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | F |
| 目标/特异性 | Nur77 (D63C5) XP® Rabbit mAb (PE Conjugate) detects endogenous levels of total human Nur77 protein. |
| 使用方法 | F(1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Nur77, also known as TR3 and NGFI-B, is an immediate-early response gene and an orphan member of the steroid/thyroid/retinoid receptor superfamily (1-3). Nur77 is composed of an amino-terminal transactivation domain, a central DNA-binding domain and a carboxy-terminal ligand-binding domain. Expression of Nur77 is rapidly induced by a variety of stimuli, including apoptotic, mitogenic and stress signals (1-6). It has been proposed to have many functions related to cell proliferation, differentiation and apoptosis. Nur77 has been extensively studied in T cells where it has been implicated in the process of negative selection and TCR-mediated apoptosis (5,6). Nur77 binds to specific DNA elements leading to the regulation of target genes (7). As a possible mechanism for regulating apoptosis, Nur77 can induce the expression of apoptotic genes such as FasL and TRAIL (8,9). Nur77 is heavily phosphorylated by multiple kinases, which may affect its transactivation activity as well as its subcellular localization (4,10,11). Translocation of Nur77 from the nucleus to the mitochondria can regulate its association with Bcl-2 and control the release of cytochrome c, thereby triggering apoptosis (12,13). |
| 存放说明 | 4C |
| 参考文献 | 1 . Hazel, T.G. et al. (1988) Proc. Natl. Acad. Sci. USA 85, 8444-8448. 2 . Chang, C. and Kokontis, J. (1988) Biochem. Biophys. Res. Commun. 155, 971-977. 3 . Milbrandt, J. (1988) Neuron 1, 183-188. 4 . Fahrner, T.J. et al. (1990) Mol. Cell. Biol. 10, 6454-6459. 5 . Liu, Z.G. et al. (1994) Nature 367, 281-284. 6 . Woronicz, J.D. et al. (1994) Nature 367, 277-281. 7 . Wilson, T.E. et al. (1991) Science 252, 1296-1300. 8 . Weih, F. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 5533-5538. 9 . Rajpal, A. et al. (2003) EMBO J. 22, 6526-6536. 10 . Hirata, Y. et al. (1993) J. Biol. Chem. 268, 24808-24812. 11 . Hazel, T.G. et al. (1991) Mol. Cell. Biol. 11, 3239-3246. 12 . Li, H. et al. (2000) Science 289, 1159-1164. 13 . Lin, B. et al. (2004) Cell 116, 527-540. |
Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with TPA #4174 and ionomycin #9995 (200 μM and 3 μM, 4 hr; green), using Nur77 (D63C5) XP® Rabbit mAb (PE Conjugate). |
