| 货号 | 67761S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | F |
| 目标/特异性 | TIM-3 (D5D5R™) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of total TIM-3 protein. |
| 使用方法 | F(1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-3 is an inhibitory molecule that is induced following T cell activation (1-3 ). TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer (4,5), and tumor-infiltrating T cells that coexpress PD-1 and TIM-3 exhibit the most severe exhausted phenotype (5). Tumor-infiltrating dendritic cells (DCs) also express TIM-3. TIM-3 expression on DCs was found to suppress innate immunity by reducing the immunogenicity of nucleic acids released by dying tumor cells (6). Research studies show that heterodimerization of TIM-3 with CEACAM-1 is critical for the inhibitory function of TIM-3, and co-blockade of TIM-3 and CEACAM-1 enhanced antitumor responses in a mouse model of colorectal cancer (7). In addition, blockade of TIM-3 in mouse models of autoimmunity enhanced the severity of disease (1). Finally, binding of Galectin-9 to TIM-3 expressed by Th1 cells induces T cell death (8). |
| 存放说明 | 4C |
| 参考文献 | 1 . Monney, L. et al. (2002) Nature 415, 536-41. 2 . Sánchez-Fueyo, A. et al. (2003) Nat Immunol 4, 1093-101. 3 . Sabatos, C.A. et al. (2003) Nat Immunol 4, 1102-10. 4 . Jones, R.B. et al. (2008) J Exp Med 205, 2763-79. 5 . Sakuishi, K. et al. (2010) J Exp Med 207, 2187-94. 6 . Chiba, S. et al. (2012) Nat Immunol 13, 832-42. 7 . Huang, Y.H. et al. (2015) Nature 517, 386-90. 8 . Zhu, C. et al. (2005) Nat Immunol 6, 1245-52. |
Flow cytometric analysis of Jurkat cells (blue) and primary CD4+ T cells (green) using TIM-3 (D5D5R) XP® Rabbit mAb (PE Conjugate). Controls were run on Jurkat cells (orange) and primary CD4+ T cells (red) using concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) #5742. CD4+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) #8907 (6.7 ng/ml). |
