| 货号 | 4627S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells.这种Cell Signaling Technology公司抗体偶联Alexa 荧光® 647荧光染料，并在人类细胞中做直接流式细胞仪和免疫荧光分析内部测试。 |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | IF-IC/F |
| 使用方法 | F(1:50) IF-IC (1:200) |
| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8). |
| 存放说明 | 4C |
Confocal immunofluorescent analysis of HeLa (left) and NCI-H28 cells (right) using β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor) and Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb (Alexa Fluor® 488 Conjugate) #9683 (green). Actin filaments have been labeled with DY-554 phalloidin (red). 使用β-Catenin (L54E2)Mouse mAb 鼠单抗（Alexa Fluor® 647偶联）（蓝色伪彩）和Acetyl-Histone H3 (Lys9) (C5B11)Rabbit mAb兔单抗（Alexa Fluor® 偶联）#9683（绿色）对HeLa细胞（左）和NCI-H28 细胞（右）进行激光共聚焦荧光分析。使用DY-554phalloidin（红色）标记肌动蛋白丝。 | |
Flow cytometric analysis of NCI-H28 cells (blue) and HeLa cells (green) using β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 647 Conjugate). 使用 β-Catenin (L54E2) Mouse mAb鼠单抗 (Alexa Fluor® 647 偶联)对NCI-H28细胞（蓝色）和HeLa细胞（绿色）进行流式分析。 |
