| 货号 | 2849S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated β-Catenin (L54E2) Mouse mAb (IF Preferred) #2677. |
| 反应种属 | Human/Rat |
| 来源宿主 | Mouse |
| 应用 | IF-F/IF-IC/F |
| 使用方法 | F(1:50) IF-F (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8). |
| 存放说明 | 4C |
Flow cytometric analysis of NCI-H28 cells (blue) and HeLa cells (green) using β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 488 Conjugate). 使用β-Catenin (L54E2) Mouse mAb鼠单抗(Alexa Fluor® 488 偶联)对NCI-H28细胞（蓝色）和HeLa（绿色）进行流式细胞分析。 | |
Confocal immunofluorescent analysis of HeLa cells using β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 488 Conjugate (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用 β-Catenin (L54E2) Mouse mAb鼠单抗 （Alexa Fluor® 488 偶联）（绿色）对HeLa细胞进行激光共聚焦荧光分析。使用DY-554phalloidin（红色）标记肌动蛋白丝。蓝色伪彩=DRAQ5®（DNA荧光染料）。 | |
Confocal immunofluorescent analysis of rat heart using β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 488 Conjugate (green). and Desmin (D93F5) XP® Rabbit mAb (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用 β-Catenin (L54E2) Mouse mAb鼠单抗 （Alexa Fluor® 488 偶联）（绿色）和Desmin (D93F5) XP® Rabbit mAb (红色)对大鼠心脏细胞进行激光共聚焦荧光分析。蓝色伪彩=DRAQ5®（DNA荧光染料）。 |
